Demonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon.
MetadataShow full item record
OBJECTIVES: The aim of this study was to investigate the transfer of bacterial doxycycline resistance between oral bacteria in subjects receiving systemic doxycycline for the treatment of periodontitis. PATIENTS AND METHODS: Streptococci were cultured before and after treatment from the subgingival plaque of two patients with periodontitis, genotyped and investigated for the presence of antimicrobial resistance determinants and conjugative transposons. RESULTS: In one subject, a strain of Streptococcus sanguinis resistant to doxycycline was a minor component of the pre-treatment streptococcal flora but dominated post-treatment. In a second subject, a strain of Streptococcus cristatus, which was sensitive to doxycycline before treatment, was found to have acquired a novel conjugative transposon during treatment, rendering it resistant to doxycycline and erythromycin. The novel transposon, named CTn6002, was sequenced and found to be a complex element derived in part from Tn916, and an unknown element which included the erythromycin resistance gene erm(B). A strain of Streptococcus oralis isolated from this subject pre-treatment was found to harbour CTn6002 and was therefore implicated as the donor. CONCLUSIONS: This is the first direct demonstration of transfer of antimicrobial resistance carried on a conjugative transposon between oral bacteria during systemic antimicrobial treatment of periodontitis in humans.
Place of Publication
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Insights into a multidrug resistant Escherichia coli pathogen of the globally disseminated ST131 lineage: genome analysis and virulence mechanisms. Totsika, M; Beatson, SA; Sarkar, S; Phan, M-D; Petty, NK; Bachmann, N; Szubert, M; Sidjabat, HE; Paterson, DL; Upton, M; Schembri, MA (United States, 2011)Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. coli clone O25b:H4-ST131 has recently emerged globally as a leading multi-drug resistant ...
The complete genome sequence of Escherichia coli EC958: a high quality reference sequence for the globally disseminated multidrug resistant E. coli O25b:H4-ST131 clone. Forde, BM; Ben Zakour, NL; Stanton-Cook, M; Phan, M-D; Totsika, M; Peters, KM; Chan, KG; Schembri, MA; Upton, M; Beatson, SA (United States, 2014)Escherichia coli ST131 is now recognised as a leading contributor to urinary tract and bloodstream infections in both community and clinical settings. Here we present the complete, annotated genome of E. coli EC958, which ...
Steglich, C; Lindell, D; Futschik, M; Rector, T; Steen, R; Chisholm, SW (England, 2010)BACKGROUND: RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, ...