Demonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon
dc.contributor.author | Warburton, Philip | |
dc.contributor.author | Palmer, RM | |
dc.contributor.author | Munson, MA | |
dc.contributor.author | Wade, WG | |
dc.date.accessioned | 2015-10-15T11:03:10Z | |
dc.date.available | 2015-10-15T11:03:10Z | |
dc.date.issued | 2007-11 | |
dc.identifier.issn | 0305-7453 | |
dc.identifier.issn | 1460-2091 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/3640 | |
dc.description.abstract |
OBJECTIVES: The aim of this study was to investigate the transfer of bacterial doxycycline resistance between oral bacteria in subjects receiving systemic doxycycline for the treatment of periodontitis. PATIENTS AND METHODS: Streptococci were cultured before and after treatment from the subgingival plaque of two patients with periodontitis, genotyped and investigated for the presence of antimicrobial resistance determinants and conjugative transposons. RESULTS: In one subject, a strain of Streptococcus sanguinis resistant to doxycycline was a minor component of the pre-treatment streptococcal flora but dominated post-treatment. In a second subject, a strain of Streptococcus cristatus, which was sensitive to doxycycline before treatment, was found to have acquired a novel conjugative transposon during treatment, rendering it resistant to doxycycline and erythromycin. The novel transposon, named CTn6002, was sequenced and found to be a complex element derived in part from Tn916, and an unknown element which included the erythromycin resistance gene erm(B). A strain of Streptococcus oralis isolated from this subject pre-treatment was found to harbour CTn6002 and was therefore implicated as the donor. CONCLUSIONS: This is the first direct demonstration of transfer of antimicrobial resistance carried on a conjugative transposon between oral bacteria during systemic antimicrobial treatment of periodontitis in humans. | |
dc.format.extent | 973-980 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press (OUP) | |
dc.subject | transposon | |
dc.subject | tetracycline | |
dc.subject | antimicrobial resistance | |
dc.subject | periodontitis | |
dc.title | Demonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000250682100009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 5 | |
plymouth.volume | 60 | |
plymouth.publication-status | Published online | |
plymouth.journal | Journal of Antimicrobial Chemotherapy | |
dc.identifier.doi | 10.1093/jac/dkm331 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | England | |
dc.identifier.eissn | 1460-2091 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1093/jac/dkm331 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review | |
plymouth.oa-location | http://jac.oxfordjournals.org/content/60/5/973.full |