A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles
dc.contributor.author | Chen, K | |
dc.contributor.author | Duong, BTV | |
dc.contributor.author | Ahmed, SU | |
dc.contributor.author | Dhavarasa, P | |
dc.contributor.author | Wang, Z | |
dc.contributor.author | Labib, M | |
dc.contributor.author | Flynn, C | |
dc.contributor.author | Xu, J | |
dc.contributor.author | Zhang, YY | |
dc.contributor.author | Wang, H | |
dc.contributor.author | Yang, X | |
dc.contributor.author | Das, J | |
dc.contributor.author | Zargartalebi, H | |
dc.contributor.author | Ma, Y | |
dc.contributor.author | Kelley, SO | |
dc.date.accessioned | 2023-09-14T09:21:09Z | |
dc.date.available | 2023-09-14T09:21:09Z | |
dc.date.issued | 2023-09-11 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.other | 5576 | |
dc.identifier.uri | https://pearl.plymouth.ac.uk/handle/10026.1/21311 | |
dc.description.abstract |
Exosomal PD-L1 (exoPD-L1) has recently received significant attention as a biomarker predicting immunotherapeutic responses involving the PD1/PD-L1 pathway. However, current technologies for exosomal analysis rely primarily on bulk measurements that do not consider the heterogeneity found within exosomal subpopulations. Here, we present a nanoscale cytometry platform NanoEPIC, enabling phenotypic sorting and exoPD-L1 profiling from blood plasma. We highlight the efficacy of NanoEPIC in monitoring anti-PD-1 immunotherapy through the interrogation of exoPD-L1. NanoEPIC generates signature exoPD-L1 patterns in responders and non-responders. In mice treated with PD1-targeted immunotherapy, exoPD-L1 is correlated with tumor growth, PD-L1 burden in tumors, and the immune suppression of CD8+ tumor-infiltrating lymphocytes. Small extracellular vesicles (sEVs) with different PD-L1 expression levels display distinctive inhibitory effects on CD8 + T cells. NanoEPIC offers robust, high-throughput profiling of exosomal markers, enabling sEV subpopulation analysis. This platform holds the potential for enhanced cancer screening, personalized treatment, and therapeutic response monitoring. | |
dc.format.extent | 5576- | |
dc.format.medium | Electronic | |
dc.language | en | |
dc.publisher | Nature Research | |
dc.subject | Animals | |
dc.subject | Mice | |
dc.subject | B7-H1 Antigen | |
dc.subject | CD8-Positive T-Lymphocytes | |
dc.subject | Cell Movement | |
dc.subject | Extracellular Vesicles | |
dc.subject | Immunosuppression Therapy | |
dc.title | A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles | |
dc.type | journal-article | |
dc.type | Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/37696888 | |
plymouth.issue | 1 | |
plymouth.volume | 14 | |
plymouth.publication-status | Published online | |
plymouth.journal | Nature Communications | |
dc.identifier.doi | 10.1038/s41467-023-41285-8 | |
plymouth.organisational-group | |Plymouth | |
plymouth.organisational-group | |Plymouth|Faculty of Health | |
plymouth.organisational-group | |Plymouth|REF 2021 Researchers by UoA | |
plymouth.organisational-group | |Plymouth|Users by role | |
plymouth.organisational-group | |Plymouth|Users by role|Academics | |
plymouth.organisational-group | |Plymouth|REF 2021 Researchers by UoA|UoA01 Clinical Medicine | |
plymouth.organisational-group | |Plymouth|Faculty of Health|Peninsula Medical School | |
dc.publisher.place | England | |
dcterms.dateAccepted | 2023-08-30 | |
dc.date.updated | 2023-09-14T09:21:02Z | |
dc.rights.embargodate | 2023-9-15 | |
dc.identifier.eissn | 2041-1723 | |
dc.rights.embargoperiod | forever | |
rioxxterms.versionofrecord | 10.1038/s41467-023-41285-8 |