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dc.contributor.authorFreitas Leal, JKen
dc.contributor.authorLasonder, Een
dc.contributor.authorSharma, Ven
dc.contributor.authorSchiller, Jen
dc.contributor.authorFanelli, Gen
dc.contributor.authorRinalducci, Sen
dc.contributor.authorBrock, Ren
dc.contributor.authorBosman, Gen
dc.date.accessioned2020-04-11T12:31:39Z
dc.date.available2020-04-11T12:31:39Z
dc.date.issued2020-03-31en
dc.identifier.urihttp://hdl.handle.net/10026.1/15555
dc.descriptionNo embargo required.en
dc.description.abstract

<jats:p>Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanisms of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic, and metabolomic analyses to obtain an integrated understanding of the mechanisms underlying microvesicle generation during the storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells.</jats:p>

en
dc.format.extent6 - 6en
dc.languageenen
dc.language.isoenen
dc.publisherMDPI AGen
dc.titleVesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequencesen
dc.typeJournal Article
plymouth.issue2en
plymouth.volume8en
plymouth.journalProteomesen
dc.identifier.doi10.3390/proteomes8020006en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dcterms.dateAccepted2020-03-28en
dc.rights.embargodate2020-06-06en
dc.identifier.eissn2227-7382en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.3390/proteomes8020006en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-03-31en
rioxxterms.typeJournal Article/Reviewen


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