Abstract
After 24h of exposure to acidic media, pyrite generates reactive oxygen species (ROS). Freshly-crushed pyrite with grain sizes between 50-100 µm at a 5 % (w/v), pulp density generated 0.17 ± 0.01 mM H2O2, while 10% pyrite generated 0.29 ± 0.01 mM and 30 % pyrite generated approximately 0.83 ± 0.06 mM. These levels of H2O2 inhibit iron oxidation in iron-grown cells of Acidithiobacillus ferrooxidansT but not in pyrite-grown cells. ROS originating from pyrite, which was incubated for 24 h in acidic medium, prohibited pyrite dissolution by iron-grown cells, while pyrite-grown cells were adapted to these concentrations of ROS. Periodical addition of 100 µM H2O2 to pyrite cultures inoculated with pyrite-grown cells did not lower iron dissolution as it was observed with iron-grown cells. By high throughput proteomics analysis, an increased expression of proteins related to oxidative stress management, iron-and sulfur oxidation systems, carbon fixation and biofilm formation was observed in biofilm cells grown on pyrite compared to iron-grown cells.
Publication Date
2017-08-01
Publication Title
Solid State Phenomena
Volume
262
Publisher
Trans Tech Publications
ISSN
1662-9779
Embargo Period
2024-11-19
First Page
372
Last Page
375
Recommended Citation
Huynh, D., Bellenberg, S., Vera Véliz, M., Poetsch, A., & Sand, W. (2017) 'Bioleaching of Pyrite by Iron-Oxidizing Acidophiles under the Influence of Reactive Oxygen Species', Solid State Phenomena, 262, pp. 372-375. Trans Tech Publications: Available at: 10.4028/www.scientific.net/SSP.262.372
