ORCID
- Upton, Mathew: 0000-0003-4287-6396
Abstract
Author Summary
The emergence and rapid dissemination of new bacterial pathogens presents multiple challenges to healthcare systems, including the need for rapid detection, precise diagnostics, effective transmission control and effective treatment. E. coli ST131 is an example of a recently emerged multidrug resistant pathogen that is capable of causing urinary tract and bloodstream infections with limited available treatment options. In order to increase our molecular understanding of E. coli ST131, we developed a high-throughput transposon mutagenesis system in combination with next generation sequencing to test every gene for its essential role in growth and for its contribution to serum resistance. We identified 315 essential genes, 270 of which were conserved among all currently available complete E. coli genomes. Fifty-six genes that define the serum resistome of E. coli ST131 were identified, including genes encoding membrane proteins, proteins involved in LPS biosynthesis, regulators and several novel proteins with previously unknown function. This study therefore provides an inventory of essential and serum resistance genes that could form a framework for the future development of targeted therapeutics to prevent disease caused by multidrug-resistant E. coli ST131 strains.
DOI
10.1371/jourl.pgen.1003834
Publication Date
2013-10-03
Publication Title
PLoS Genet
Volume
9
Organisational Unit
School of Biomedical Sciences
First Page
e1003834
Last Page
e1003834
Recommended Citation
Phan, M. D., Peters, K., Sarkar, S., Lukowski, S., Allsopp, L., Moriel, D., Achard, M., Totsika, M., Marshall, V., Upton, M., Beatson, S., & Schembri, M. (2013) 'The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone', PLoS Genet, 9, pp. e1003834-e1003834. Available at: https://doi.org/10.1371/jourl.pgen.1003834
