Abstract

Porphyromonas gingivalis produces different LPS isoforms with significant structural variations of their lipid A and O-antigen moieties that can affect its pro-inflammatory and bone-resorbing potential. We show here, for the first time, that P. gingivalis LPS isolated from W83 strain is highly sialylated and possesses significantly reduced inflammatory potential compared with less sialylated ATCC 33277 strain LPS. Nevertheless, the reduction in the endotoxin activity is not mediated by the presence of sialic acid LPS moieties as the sialic acid-free LPS produced by the mutant W83 strain exhibits a similar inflammatory potential to the wild type strain. Furthermore, our findings suggest that the interaction between the sialic acid LPS moieties and the inhibitory CD33 receptor is prevented by endogenously expressed sialic acid on the surface of THP-1 cells that cannot be out-competed by sialic acid containing P. gingivalis LPS. The present study also highlights the importance of endogenous sialic acid as a 'self-associated molecular pattern' and CD33 receptors in modulation of innate immune response as human gingival fibroblasts, which do not express CD33 receptors, and desialylated THP-1 cells have both been found to have much higher spontaneous IL-8 production than naïve THP-1 cells.

Publication Date

2017-01-01

Publication Title

Innate Immunity

Volume

23

Issue

3

ISSN

1753-4259

Keywords

Monocytes, Cell Line, Fibroblasts, Gingiva, Humans, Porphyromonas gingivalis, Bacteroidaceae Infections, Periodontal Diseases, N-Acetylneuraminic Acid, Lipopolysaccharides, Lipid A, O Antigens, Interleukin-8, Protein Processing, Post-Translational, Mutation, Host-Pathogen Interactions, Immunity, Innate, Sialic Acid Binding Ig-like Lectin 3

First Page

319

Last Page

326

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10.1177/1753425917694245" data-hide-no-mentions="true">

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