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dc.contributor.authorLasonder, Een
dc.contributor.authorJanse, CJen
dc.contributor.authorvan Gemert, G-Jen
dc.contributor.authorMair, GRen
dc.contributor.authorVermunt, AMWen
dc.contributor.authorDouradinha, BGen
dc.contributor.authorvan Noort, Ven
dc.contributor.authorHuynen, MAen
dc.contributor.authorLuty, AJFen
dc.contributor.authorKroeze, Hen
dc.contributor.authorKhan, SMen
dc.contributor.authorSauerwein, RWen
dc.contributor.authorWaters, APen
dc.contributor.authorMann, Men
dc.contributor.authorStunnenberg, HGen
dc.date.accessioned2016-07-11T15:05:38Z
dc.date.available2016-07-11T15:05:38Z
dc.date.issued2008-10en
dc.identifier.urihttp://hdl.handle.net/10026.1/5047
dc.description.abstract

Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito -- early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.

en
dc.format.extente1000195 - ?en
dc.languageengen
dc.language.isoengen
dc.subjectAnimalsen
dc.subjectAnophelesen
dc.subjectDatabases, Geneticen
dc.subjectHumansen
dc.subjectMalaria, Falciparumen
dc.subjectMiceen
dc.subjectMice, Knockouten
dc.subjectOocystsen
dc.subjectPlasmodium bergheien
dc.subjectPlasmodium falciparumen
dc.subjectProteomeen
dc.subjectProteomicsen
dc.subjectProtozoan Proteinsen
dc.subjectSalivary Glandsen
dc.subjectSporozoitesen
dc.titleProteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/18974882en
plymouth.issue10en
plymouth.volume4en
plymouth.publication-statusPublisheden
plymouth.journalPLoS Pathogen
dc.identifier.doi10.1371/journal.ppat.1000195en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group/RC Reporting Group BRG
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
dc.publisher.placeUnited Statesen
dcterms.dateAccepted2008-10-09en
dc.identifier.eissn1553-7374en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1371/journal.ppat.1000195en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2008-10en
rioxxterms.typeJournal Article/Reviewen


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