Show simple item record

dc.contributor.authorLasonder, Een
dc.contributor.authorRijpma, SRen
dc.contributor.authorvan Schaijk, BCen
dc.contributor.authorHoeijmakers, WAen
dc.contributor.authorKensche, PRen
dc.contributor.authorGresnigt, MSen
dc.contributor.authorItaliaander, Aen
dc.contributor.authorVos, MWen
dc.contributor.authorWoestenenk, Ren
dc.contributor.authorBousema, Ten
dc.contributor.authorMair, GRen
dc.contributor.authorKhan, SMen
dc.contributor.authorJanse, CJen
dc.contributor.authorBártfai, Ren
dc.contributor.authorSauerwein, RWen
dc.date.accessioned2016-06-23T09:12:07Z
dc.date.available2016-06-23T09:12:07Z
dc.date.issued2016-07-27en
dc.identifier.issn0305-1048en
dc.identifier.urihttp://hdl.handle.net/10026.1/4954
dc.description.abstract

Sexual differentiation of malaria parasites into gametocytes in the vertebrate host and subsequent gamete fertilization in mosquitoes is essential for the spreading of the disease. The molecular processes orchestrating these transitions are far from fully understood. Here, we report the first transcriptome analysis of male and female Plasmodium falciparum gametocytes coupled with a comprehensive proteome analysis. In male gametocytes there is an enrichment of proteins involved in the formation of flagellated gametes; proteins involved in DNA replication, chromatin organization and axoneme formation. On the other hand, female gametocytes are enriched in proteins required for zygote formation and functions after fertilization; protein-, lipid- and energy-metabolism. Integration of transcriptome and proteome data revealed 512 highly expressed maternal transcripts without corresponding protein expression indicating large scale translational repression in P. falciparum female gametocytes for the first time. Despite a high degree of conservation between Plasmodium species, 260 of these 'repressed transcripts' have not been previously described. Moreover, for some of these genes, protein expression is only reported in oocysts and sporozoites indicating that repressed transcripts can be partitioned into short- and long-term storage. Finally, these data sets provide an essential resource for identification of vaccine/drug targets and for further mechanistic studies.

en
dc.language.isoenen
dc.titleIntegrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression.en
dc.typeJournal Article
plymouth.journalNucleic acids researchen
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group/RC Reporting Group BRG
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
dcterms.dateAccepted2016-06-06en
dc.identifier.eissn1362-4962en
dc.rights.embargoperiodNot knownen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-07-27en
rioxxterms.typeJournal Article/Reviewen
plymouth.oa-locationhttps://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw536en


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
@mire NV