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dc.contributor.authorLasonder, E
dc.contributor.authorRijpma, SR
dc.contributor.authorvan Schaijk, BC
dc.contributor.authorHoeijmakers, WA
dc.contributor.authorKensche, PR
dc.contributor.authorGresnigt, MS
dc.contributor.authorItaliaander, A
dc.contributor.authorVos, MW
dc.contributor.authorWoestenenk, R
dc.contributor.authorBousema, T
dc.contributor.authorMair, GR
dc.contributor.authorKhan, SM
dc.contributor.authorJanse, CJ
dc.contributor.authorBártfai, R
dc.contributor.authorSauerwein, RW
dc.date.accessioned2016-06-23T09:12:07Z
dc.date.available2016-06-23T09:12:07Z
dc.date.issued2016-07-27
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.urihttp://hdl.handle.net/10026.1/4954
dc.description.abstract

Sexual differentiation of malaria parasites into gametocytes in the vertebrate host and subsequent gamete fertilization in mosquitoes is essential for the spreading of the disease. The molecular processes orchestrating these transitions are far from fully understood. Here, we report the first transcriptome analysis of male and female Plasmodium falciparum gametocytes coupled with a comprehensive proteome analysis. In male gametocytes there is an enrichment of proteins involved in the formation of flagellated gametes; proteins involved in DNA replication, chromatin organization and axoneme formation. On the other hand, female gametocytes are enriched in proteins required for zygote formation and functions after fertilization; protein-, lipid- and energy-metabolism. Integration of transcriptome and proteome data revealed 512 highly expressed maternal transcripts without corresponding protein expression indicating large scale translational repression in P. falciparum female gametocytes for the first time. Despite a high degree of conservation between Plasmodium species, 260 of these 'repressed transcripts' have not been previously described. Moreover, for some of these genes, protein expression is only reported in oocysts and sporozoites indicating that repressed transcripts can be partitioned into short- and long-term storage. Finally, these data sets provide an essential resource for identification of vaccine/drug targets and for further mechanistic studies.

dc.format.extent6087-6101
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subjectChromatin
dc.subjectDNA Replication
dc.subjectFemale
dc.subjectGametogenesis
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectMalaria, Falciparum
dc.subjectMale
dc.subjectMetabolic Networks and Pathways
dc.subjectPlasmodium falciparum
dc.subjectProtein Biosynthesis
dc.subjectProteome
dc.subjectSex Characteristics
dc.subjectTranscriptome
dc.titleIntegrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression.
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27298255
plymouth.issue13
plymouth.volume44
plymouth.publication-statusPublished
plymouth.journalNucleic acids research
dc.identifier.doi10.1093/nar/gkw536
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
dc.publisher.placeEngland
dcterms.dateAccepted2016-06-06
dc.identifier.eissn1362-4962
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1093/nar/gkw536
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2016-07-27
rioxxterms.typeJournal Article/Review
plymouth.oa-locationhttps://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw536


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