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dc.contributor.authorWarburton, PJ
dc.contributor.authorAllan, E
dc.contributor.authorHunter, S
dc.contributor.authorWard, J
dc.contributor.authorBooth, V
dc.contributor.authorWade, WG
dc.contributor.authorMullany, P
dc.date.accessioned2015-10-14T11:33:21Z
dc.date.available2015-10-14T11:33:21Z
dc.date.issued2011-11
dc.identifier.issn0168-6496
dc.identifier.issn1574-6941
dc.identifier.urihttp://hdl.handle.net/10026.1/3626
dc.description.abstract

The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.

dc.format.extent349-354
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherOxford University Press (OUP)
dc.subjectoral cavity
dc.subjectplasmid
dc.subjectTRACA
dc.subjectdental plaque
dc.subjectmetagenome
dc.titleIsolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA)
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/21711368
plymouth.issue2
plymouth.volume78
plymouth.publisher-urlhttp://dx.doi.org/10.1111/j.1574-6941.2011.01166.x
plymouth.publication-statusPublished
plymouth.journalFEMS Microbiology Ecology
dc.identifier.doi10.1111/j.1574-6941.2011.01166.x
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeEngland
dc.identifier.eissn1574-6941
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1111/j.1574-6941.2011.01166.x
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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