Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA)
dc.contributor.author | Warburton, PJ | |
dc.contributor.author | Allan, E | |
dc.contributor.author | Hunter, S | |
dc.contributor.author | Ward, J | |
dc.contributor.author | Booth, V | |
dc.contributor.author | Wade, WG | |
dc.contributor.author | Mullany, P | |
dc.date.accessioned | 2015-10-14T11:33:21Z | |
dc.date.available | 2015-10-14T11:33:21Z | |
dc.date.issued | 2011-11 | |
dc.identifier.issn | 0168-6496 | |
dc.identifier.issn | 1574-6941 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/3626 | |
dc.description.abstract |
The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated. | |
dc.format.extent | 349-354 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press (OUP) | |
dc.subject | oral cavity | |
dc.subject | plasmid | |
dc.subject | TRACA | |
dc.subject | dental plaque | |
dc.subject | metagenome | |
dc.title | Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA) | |
dc.type | journal-article | |
dc.type | Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/21711368 | |
plymouth.issue | 2 | |
plymouth.volume | 78 | |
plymouth.publication-status | Published | |
plymouth.journal | FEMS Microbiology Ecology | |
dc.identifier.doi | 10.1111/j.1574-6941.2011.01166.x | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | England | |
dc.identifier.eissn | 1574-6941 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1111/j.1574-6941.2011.01166.x | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review |