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dc.contributor.authorDesousa, BR
dc.contributor.authorKim, KKO
dc.contributor.authorJones, AE
dc.contributor.authorBall, AB
dc.contributor.authorHsieh, WY
dc.contributor.authorSwain, P
dc.contributor.authorMorrow, DH
dc.contributor.authorBrownstein, AJ
dc.contributor.authorFerrick, DA
dc.contributor.authorShirihai, OS
dc.contributor.authorNeilson, A
dc.contributor.authorNathanson, DA
dc.contributor.authorRogers, GW
dc.contributor.authorDranka, BP
dc.contributor.authorMurphy, AN
dc.contributor.authorAffourtit, Charles
dc.contributor.authorBensinger, SJ
dc.contributor.authorStiles, L
dc.contributor.authorRomero, N
dc.contributor.authorDivakaruni, AS
dc.date.accessioned2023-08-08T11:04:28Z
dc.date.available2023-08-08T11:04:28Z
dc.date.issued2023-08-07
dc.identifier.issn1469-221X
dc.identifier.issn1469-3178
dc.identifier.otherARTN e56380
dc.identifier.urihttps://pearl.plymouth.ac.uk/handle/10026.1/21182
dc.description.abstract

Oxidative phosphorylation and glycolysis are the dominant ATP-generating pathways in mammalian metabolism. The balance between these two pathways is often shifted to execute cell-specific functions in response to stimuli that promote activation, proliferation, or differentiation. However, measurement of these metabolic switches has remained mostly qualitative, making it difficult to discriminate between healthy, physiological changes in energy transduction or compensatory responses due to metabolic dysfunction. We therefore present a broadly applicable method to calculate ATP production rates from oxidative phosphorylation and glycolysis using Seahorse XF Analyzer data and empirical conversion factors. We quantify the bioenergetic changes observed during macrophage polarization as well as cancer cell adaptation to in vitro culture conditions. Additionally, we detect substantive changes in ATP utilization upon neuronal depolarization and T cell receptor activation that are not evident from steady-state ATP measurements. This method generates a single readout that allows the direct comparison of ATP produced from oxidative phosphorylation and glycolysis in live cells. Additionally, the manuscript provides a framework for tailoring the calculations to specific cell systems or experimental conditions.

dc.format.extente56380-
dc.format.mediumPrint-Electronic
dc.languageen
dc.publisherEMBO
dc.subjectATP
dc.subjectECAR
dc.subjectglycolysis
dc.subjectoxidative phosphorylation
dc.subjectSeahorse XF Analyzer
dc.titleCalculation of ATP production rates using the Seahorse XF Analyzer
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:001043137300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.publication-statusPublished online
plymouth.journalEMBO reports
dc.identifier.doi10.15252/embr.202256380
plymouth.organisational-group|Plymouth
plymouth.organisational-group|Plymouth|Research Groups
plymouth.organisational-group|Plymouth|Faculty of Health
plymouth.organisational-group|Plymouth|Research Groups|Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group|Plymouth|Research Groups|Institute of Translational and Stratified Medicine (ITSMED)|CBR
plymouth.organisational-group|Plymouth|REF 2021 Researchers by UoA
plymouth.organisational-group|Plymouth|Users by role
plymouth.organisational-group|Plymouth|Users by role|Academics
plymouth.organisational-group|Plymouth|REF 2021 Researchers by UoA|UoA03 Allied Health Professions, Dentistry, Nursing and Pharmacy
plymouth.organisational-group|Plymouth|Faculty of Health|School of Biomedical Sciences
dc.publisher.placeEngland
dcterms.dateAccepted2023-07-14
dc.date.updated2023-08-08T11:04:17Z
dc.rights.embargodate2023-8-9
dc.identifier.eissn1469-3178
dc.rights.embargoperiodforever
rioxxterms.versionofrecord10.15252/embr.202256380


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