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dc.contributor.authorTang-Huau, T-Len
dc.contributor.authorRosenke, Ken
dc.contributor.authorMeade-White, Ken
dc.contributor.authorCarmody, Aen
dc.contributor.authorSmith, BJen
dc.contributor.authorBosio, CMen
dc.contributor.authorJarvis, MAen
dc.contributor.authorFeldmann, Hen
dc.date.accessioned2021-08-09T12:35:37Z
dc.date.issued2021-04-22en
dc.identifier.issn1999-4915en
dc.identifier.urihttp://hdl.handle.net/10026.1/17494
dc.description.abstract

<jats:p>The multimammate mouse (Mastomys natalensis; M. natalensis) has been identified as a major reservoir for multiple human pathogens including Lassa virus (LASV), Leishmania spp., Yersinia spp., and Borrelia spp. Although M. natalensis are related to well-characterized mouse and rat species commonly used in laboratory models, there is an absence of established assays and reagents to study the host immune responses of M. natalensis. As a result, there are major limitations to our understanding of immunopathology and mechanisms of immunological pathogen control in this increasingly important rodent species. In the current study, a large panel of commercially available rodent reagents were screened to identify their cross-reactivity with M. natalensis. Using these reagents, ex vivo assays were established and optimized to evaluate lymphocyte proliferation and cytokine production by M. natalensis lymphocytes. In contrast to C57BL/6J mice, lymphocytes from M. natalensis were relatively non-responsive to common stimuli such as phytohaemagglutinin P and lipopolysaccharide. However, they readily responded to concanavalin A stimulation as indicated by proliferation and cytokine production. In summary, we describe lymphoproliferative and cytokine assays demonstrating that the cellular immune responses in M. natalensis to commonly used mitogens differ from a laboratory-bred mouse strain.</jats:p>

en
dc.format.extent729 - 729en
dc.languageenen
dc.language.isoenen
dc.publisherMDPIen
dc.titleMastomys natalensis Has a Cellular Immune Response Profile Distinct from Laboratory Miceen
dc.typeJournal Article
plymouth.issue5en
plymouth.volume13en
plymouth.journalVirusesen
dc.identifier.doi10.3390/v13050729en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dcterms.dateAccepted2021-04-17en
dc.rights.embargodate2021-12-03en
dc.identifier.eissn1999-4915en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.3390/v13050729en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2021-04-22en
rioxxterms.typeJournal Article/Reviewen


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