Isolation of Oropouche Virus from Febrile Patient, Ecuador

Date
2018-05Author
Wise, EL
Pullan, ST
Márquez, S
Paz, V
Mosquera, JD
Zapata, S
Jackson, SK
Trueba, G
Logue, CH
Subject
Arbovirus Bunyaviridae Ecuador Oropouche Orthobunyaviridae South America bunyavirus communicable disease high-throughput nucleotide sequencing metagenomic orthobunyavirus virus viruses Adult Animals Bunyaviridae Infections Chlorocebus aethiops Ecuador Humans Male Orthobunyavirus Phylogeny Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Vero Cells
Metadata
Show full item recordAbstract
We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.
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Publisher
Centers for Disease Control and Prevention (CDC)
Place of Publication
United States
Journal
Emerging Infectious Diseases
Volume
24
Issue
5
Pagination
935-937
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