Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli
dc.contributor.author | Dawson, SE | |
dc.contributor.author | Gibreel, T | |
dc.contributor.author | Nicolaou, N | |
dc.contributor.author | AlRabiah, H | |
dc.contributor.author | Xu, Y | |
dc.contributor.author | Goodacre, R | |
dc.contributor.author | Upton, M | |
dc.date.accessioned | 2018-03-21T09:45:41Z | |
dc.date.issued | 2014-06 | |
dc.identifier.issn | 0934-9723 | |
dc.identifier.issn | 1435-4373 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/11125 | |
dc.description.abstract |
In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs. | |
dc.format.extent | 983-988 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Springer Science and Business Media LLC | |
dc.subject | Bacterial Typing Techniques | |
dc.subject | Humans | |
dc.subject | Spectroscopy, Fourier Transform Infrared | |
dc.subject | Uropathogenic Escherichia coli | |
dc.title | Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli | |
dc.type | journal-article | |
dc.type | Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/24399364 | |
plymouth.issue | 6 | |
plymouth.volume | 33 | |
plymouth.publication-status | Published | |
plymouth.journal | European Journal of Clinical Microbiology & Infectious Diseases | |
dc.identifier.doi | 10.1007/s10096-013-2036-0 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR) | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | Germany | |
dcterms.dateAccepted | 2013-12-10 | |
dc.identifier.eissn | 1435-4373 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1007/s10096-013-2036-0 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2014-06 | |
rioxxterms.type | Journal Article/Review |