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dc.contributor.authorVoets, GMen
dc.contributor.authorLeverstein-van Hall, MAen
dc.contributor.authorKolbe-Busch, Sen
dc.contributor.authorvan der Zanden, Aen
dc.contributor.authorChurch, Den
dc.contributor.authorKaase, Men
dc.contributor.authorGrisold, Aen
dc.contributor.authorUpton, Men
dc.contributor.authorCloutman-Green, Een
dc.contributor.authorCantón, Ren
dc.contributor.authorFriedrich, AWen
dc.contributor.authorFluit, ACen
dc.contributor.authorDiversiLab Study Groupen
dc.date.accessioned2018-03-10T10:16:12Z
dc.date.available2018-03-10T10:16:12Z
dc.date.issued2013-12en
dc.identifier.urihttp://hdl.handle.net/10026.1/11056
dc.description.abstract

Successful multidrug-resistant clones are increasing in prevalence globally, which makes the ability to identify these clones urgent. However, adequate, easy-to-perform, and reproducible typing methods are lacking. We investigated whether DiversiLab (DL), an automated repetitive-sequence-based PCR bacterial typing system (bioMérieux), is suitable for comparing isolates analyzed at different geographic centers. A total of 39 Escherichia coli and 39 Klebsiella species isolates previously typed by the coordinating center were analyzed. Pulsed-field gel electrophoresis (PFGE) confirmed the presence of one cluster of 6 isolates, three clusters of 3 isolates, and three clusters of 2 isolates for each set of isolates. DL analysis was performed in 11 centers in six different countries using the same protocol. The DL profiles of 425 E. coli and 422 Klebsiella spp. were obtained. The DL system showed a lower discriminatory power for E. coli than did PFGE. The local DL data showed a low concordance, as indicated by the adjusted Rand and Wallace coefficients (0.132 to 0.740 and 0.070 to 1.0 [E. coli] and 0.091 to 0.864 and 0.056 to 1.0 [Klebsiella spp.], respectively). The central analysis showed a significantly improved concordance (0.473 to 1.0 and 0.290 to 1.0 [E. coli] and 0.513 to 0.965 and 0.425 to 1.0 [Klebsiella spp.], respectively). The misclassifications of profiles for individual isolates were mainly due to inconsistent amplification, which was most likely due to variations in the quality and amounts of the isolated DNA used for amplification. Despite local variations, the DL system has the potential to indicate the occurrence of clonal outbreaks in an international setting, provided there is strict adherence to standardized, reproducible DNA isolation methods and analysis protocols, all supported by a central database for profile comparisons.

en
dc.format.extent3944 - 3949en
dc.languageengen
dc.language.isoengen
dc.subjectDNA, Bacterialen
dc.subjectEscherichia colien
dc.subjectHumansen
dc.subjectInternational Cooperationen
dc.subjectKlebsiellaen
dc.subjectMolecular Typingen
dc.subjectPolymerase Chain Reactionen
dc.subjectReproducibility of Resultsen
dc.titleInternational multicenter evaluation of the DiversiLab bacterial typing system for Escherichia coli and Klebsiella spp.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/24025914en
plymouth.issue12en
plymouth.volume51en
plymouth.publication-statusPublisheden
plymouth.journalJ Clin Microbiolen
dc.identifier.doi10.1128/JCM.01664-13en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group/RC Reporting Group BRG
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
dc.publisher.placeUnited Statesen
dc.identifier.eissn1098-660Xen
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1128/JCM.01664-13en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


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