ORCID
- Foey, Andrew: 0000-0003-0419-2268
- Salih, Vehid: 0000-0003-3776-2941
Abstract
Early in vitro oral mucosal infection models (OMMs) failed to consider the suitability of the model environment to represent the host immune response. Denture stomatitis (DS) is mediated by Candida albicans, but the role of Staphylococcus aureus remains uncertain. A collagen hydrogel-based OMM containing HaCaT and HGF cell types was developed, characterised and employed to study of tissue invasion and pro-inflammatory cytokine production in response to pathogens. Models formed a robust epithelium. Despite their inflammatory baseline, 24-h infection with C. albicans, and/or S. aureus led to tissue invasion, and significantly upregulated IL-6 and IL-8 production by OMMs when compared to the unstimulated control. No significant difference in IL-6 or IL-8 production by OMMs was observed between single and dual infections. These attributes indicate that this newly developed OMM is suitable for the study of DS and could be implemented for the wider study of oral infection.
DOI
10.1177/20417314231197310
Publication Date
2023-10-21
Publication Title
Journal of Tissue Engineering
Volume
14
Embargo Period
2023-11-02
Organisational Unit
School of Biomedical Sciences
Recommended Citation
Gould, S. J., Foey, A., & Salih, V. (2023) 'An organotypic oral mucosal infection model to study host-pathogen interactions', Journal of Tissue Engineering, 14. Available at: https://doi.org/10.1177/20417314231197310
