Abstract
Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomic data with existing mRNA data to derive robust cell-specific expression signatures with more than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1ß secretion in response to ATP.
DOI
10.1016/j.celrep.2016.08.023
Publication Date
2016-09-13
Publication Title
Cell Rep
Volume
16
Issue
11
Publisher
Elsevier BV
ISSN
2211-1247
Embargo Period
2024-11-19
Additional Links
http://www.ncbi.nlm.nih.gov/pubmed/27626665
First Page
2953
Last Page
2966
Recommended Citation
Worah, K., Mathan, T., Vu Manh, T., Keerthikumar, S., & et al. (2016) 'Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function.', Cell Rep, 16(11), pp. 2953-2966. Elsevier BV: Available at: https://doi.org/10.1016/j.celrep.2016.08.023
