Abstract
Mini-chromosome maintenance (MCM) proteins play an essential role in DNA replication initiation. We have isolated a novel gene encoding an MCM-like protein from the human malaria parasite Plasmodium falciparum using the vectorette technique. The gene has no introns and comprises an open reading frame encoding 1005 amino acid residues with a predicted Mr of 115 kDa. The encoded protein, termed PfMCM4, contains all conserved sequences in the MCM family and displays the highest homology to the Cdc54 (MCM4) of Saccharomyces cerevisiae. However, PfMCM4 possesses five unique amino acid inserts with sizes ranging from seven to 75 residues. Southern blotting of genomic DNA digests and chromosomal separations showed that the Pfmcm4 gene is present as a single copy per haploid genome and is located on chromosome 13. A 4000-nucleotide transcript of this gene is expressed specifically in the sexual erythrocytic stage, indicating that PfMCM4 may be involved in gametogenesis in which DNA is quickly replicated.
DOI
10.1016/s0020-7519(01)00237-5
Publication Date
2001-09-01
Publication Title
International Journal for Parasitology
Volume
31
Issue
11
Publisher
Elsevier BV
ISSN
0020-7519
Embargo Period
2024-11-19
First Page
1246
Last Page
1252
Recommended Citation
Li, J., & Cox, L. (2001) 'Identification of an MCM4 homologue expressed specifically in the sexual stage of Plasmodium falciparum', International Journal for Parasitology, 31(11), pp. 1246-1252. Elsevier BV: Available at: https://doi.org/10.1016/s0020-7519(01)00237-5
