Abstract
A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome beta-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a 156-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding.
DOI
10.1016/s0020-7519(00)00046-1
Publication Date
2000-05-01
Publication Title
International Journal for Parasitology
Volume
30
Issue
6
Publisher
Elsevier BV
ISSN
0020-7519
Embargo Period
2024-11-19
First Page
729
Last Page
733
Recommended Citation
Li, G., Li, J., Mugthin, M., & Ward, S. (2000) 'Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum', International Journal for Parasitology, 30(6), pp. 729-733. Elsevier BV: Available at: https://doi.org/10.1016/s0020-7519(00)00046-1
