ORCID

Abstract

This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.

DOI

10.3390/s141120519

Publication Date

2014-10-30

Publication Title

Sensors (Switzerland)

Volume

14

Issue

11

ISSN

1424-8220

Keywords

96-well plate, Amperometry, Cell monitoring, MEMS, Metabolism, Microbiosensor, Screen-printing, Toxicity testing

First Page

20519

Last Page

20532

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