ORCID
- Affourtit, Charles: 0000-0003-1776-9943
Abstract
Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton leaks. In this chapter, we present a method to measure proton leak activity in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasized.
DOI
10.1007/978-1-4939-7831-1_9
Publication Date
2018-05-23
Publication Title
Methods in Molecular Biology
Volume
1782
ISSN
1064-3745
Embargo Period
2019-05-23
Organisational Unit
School of Biomedical Sciences
Keywords
Membrane potential, Mitochondria, Modular kinetic analysis, Oxidative phosphorylation, Oxygen consumption
First Page
157
Last Page
170
Recommended Citation
Affourtit, C., Wong, H., & Brand, M. (2018) 'Measurement of Proton Leak in Isolated Mitochondria.', Methods in Molecular Biology, 1782, pp. 157-170. Available at: https://doi.org/10.1007/978-1-4939-7831-1_9
