ORCID
- Warburton, Philip: 0000-0002-5810-0296
- Howell, Kerry: 0000-0003-3359-1778
- Upton, Mathew: 0000-0003-4287-6396
Abstract
Access to deep-sea sponges brings with it the potential to discover novel antimicrobial candidates, as well as novel cold- and pressure-adapted bacteria with further potential clinical or industrial applications. In this study, we implemented a combination of different growth media, increased pressure and high-throughput techniques to optimize recovery of isolates from two deep-sea hexactinellid sponges, Pheronema carpenteri and Hertwigia sp., in the first culture-based microbial analysis of these two sponges. Using 16S rRNA gene sequencing for isolate identification, we found a similar number of cultivable taxa from each sponge species, as well as improved recovery of morphotypes from P. carpenteri at 22–25 °C compared to other temperatures, which allows a greater potential for screening for novel antimicrobial compounds. Bacteria recovered under conditions of increased pressure were from the phyla Proteobacteria, Actinobacteria and Firmicutes, except at 4 %O2/5 bar, when the phylum Firmicutes was not observed. Cultured isolates from both sponge species displayed antimicrobial activity against Micrococcus luteus, Staphylococcus aureus and Escherichia coli.
DOI
10.1099/mic.0.001123
Publication Date
2021-12-13
Publication Title
Microbiology
Volume
167
Issue
12
ISSN
1350-0872
Embargo Period
2022-01-06
Organisational Unit
School of Biomedical Sciences
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Recommended Citation
Koch, M. J., Hesketh-Best, P., Smerdon, G., Warburton, P., Howell, K., & Upton, M. (2021) 'Impact of growth media and pressure on the diversity and antimicrobial activity of isolates from two species of hexactinellid sponge', Microbiology, 167(12). Available at: https://doi.org/10.1099/mic.0.001123
