ORCID
- Upton, Mathew: 0000-0003-4287-6396
- Jarvis, Michael: 0000-0002-0124-4061
Abstract
Streptococcus suis (S. suis) is a bacterial pathogen of pigs that has a major animal health and economic impact on the pig industry. Bovine herpesvirus-4 (BoHV-4) is a new virus-based vaccine vector that has been used for the immunogenic delivery of antigens from a variety of pathogens. In the present study, two recombinant BoHV-4-based vectors were evaluated for their ability to induce immunity and protection against S. suis in a rabbit model. The GMD protein is a fusion protein consisting of multiple dominant B-cell epitopes ((B-cell dominant epitopes of GAPDH, MRP, and DLDH antigens) (BoHV-4/GMD)) and the second suilysin (SLY) (BoHV-4/SLY) from S. suis serotype 2 (SS2). Both GMD and SLY delivered by the BoHV-4 vectors were recognized by sera from SS2-infected rabbits. The vaccination of rabbits with the BoHV-4 vectors induced antibodies against SS2, as well as against additional S. suis serotypes, SS7 and SS9. However, sera from BoHV-4/GMD-vaccinated animals promoted a significant level of phagocytic activity by pulmonary alveolar macrophages (PAMs) against SS2, SS7, and SS9. In contrast, sera from rabbits immunized with BoHV-4/SLY induced PAM phagocytic activity against only SS2. In addition, BoHV-4 vaccines differed in the associated level of protection against lethal SS2 challenge, which ranged from high (71.4%) to low (12.5%) for BoHV-4/GMD and BoHV-4/SLY, respectively. These data suggest BoHV-4/GMD as a promising vaccine candidate against S. suis disease.
DOI
10.3390/vaccines11051004
Publication Date
2023-05-20
Publication Title
Vaccines
Volume
11
Issue
5
ISSN
2076-393X
Embargo Period
2023-06-10
Organisational Unit
School of Biomedical Sciences
Recommended Citation
Dong, N., Nichols, H., Sun, Q., Chen, X., Zheng, J., Guan, Z., Zhang, H., Davison, A., Wezel, Y., Li, Z., Li, B., Liu, K., Shao, D., Qiu, Y., Sun, J., Li, X., Upton, M., Ma, Z., Jarvis, M., & Wei, J. (2023) 'Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model', Vaccines, 11(5). Available at: https://doi.org/10.3390/vaccines11051004
