ORCID
- Upton, Mathew: 0000-0003-4287-6396
Abstract
In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.
DOI
10.1007/s10096-013-2036-0
Publication Date
2013-12-10
Publication Title
Eur J Clin Microbiol Infect Dis
Volume
33
Issue
6
First Page
983
Last Page
988
Organisational Unit
School of Biomedical Sciences
Keywords
Bacterial Typing Techniques, Humans, Spectroscopy, Fourier Transform Infrared, Uropathogenic Escherichia coli
Recommended Citation
Dawson, S. E., Gibreel, T., Nicolaou, N., AlRabiah, H., Xu, Y., Goodacre, R., & Upton, M. (2013) 'Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.', Eur J Clin Microbiol Infect Dis, 33(6), pp. 983-988. Available at: https://doi.org/10.1007/s10096-013-2036-0
