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The Plymouth Student Scientist

Document Type

Biological and Marine Sciences Article

Abstract

Background and purpose Nitric oxide (NO) has been well documented as a potent, endogenous vasodilator. The effects of NO within the pulmonary and systemic vasculature have been investigated to some degree but the mechanisms behind its regulation are not fully understood. Mitochondria have been shown to regulate potassium channels that are important in the regulation of vascular tone. This study aims to observe the effects of mitochondrial inhibition of the electron transport chain at different complexes and within different vascular tissues to further elucidate this system. Experimental approach I examined the effects of mitochondrial transport chain inhibitors rotenone (inhibition at complex 3) and antimysin-A (inhibition at complex 1) on the relaxation of rat aortic and pulmonary vessels, induced by sodium nitroprusside (SNP). Key results Inhibition of mitochondrial electron transport chain at complex 1 surprisingly caused a great increase in levels of vasodilation within the pulmonary artery. Inhibition of mitochondrial electron transport chain at complex 1 also caused an increase of SNP induced vasodilation within the aorta. Inhibition of the mitochondrial electron transport chain at complex 3 resulted in a markedly decreased vasodilatory effect of SNP on the aorta. Conclusions and implications My results demonstrate the importance of mitochondrial activity in the regulation of NO's vasodilator action within the pulmonary and systemic vasculature. It also highlights the relevance of activity at certain complexes within the mitochondrial electron transport chain, suggesting a strong correlation between redox state and co-localised potassium channels. This promotes further research into the specificity of activity at certain complexes and the potential for further development of therapeutic NO donors in the treatment of cardiovascular disorders.

Publication Date

2013-07-01

Publication Title

The Plymouth Student Scientist

Volume

6

Issue

1

First Page

16

Last Page

31

ISSN

1754-2383

Deposit Date

May 2019

Embargo Period

2024-07-03

URI

http://hdl.handle.net/10026.1/14006

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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