Functionalization of Graphene Oxide for Label-Free Electrochemical Detection of Hepatic Cancer Cells
ORCID
- Shakil A. Awan: 0000-0002-8543-3328
Abstract
: In this study, a graphene oxide-modified screen-printed carbon electrode was functionalized with the 1-pyrenebutyric acid-N-hydroxy-succinimide ester and conjugated with antibodies for the label-free detection of human hepatoma HepG2 cells. Using a user-friendly reservoir chamber, the functionalized film was exposed continuously to the cancer cells. The use of a continuous flow was intended to enhance the capture of the target cells by the sensing platform. The response of the biosensor was evaluated using cyclic voltammetry. The preliminary data showed good sensitivity for the detection of hepatic cancer cells. The developed biosensor could detect the HepG2 cells from a 1 × 103 to 3 × 105 cells/mL range. Thus, it is a simple tool for the electrochemical detection of cancer cells and offers a low-cost and disposable sensing platform.
DOI Link
Publication Date
2023-05-12
Event
3rd International Electronic Conference on Biosensors IECB
Publication Title
Engineering Proceedings
Volume
35
Issue
1
Acceptance Date
2023-05-12
Deposit Date
2023-10-08
Recommended Citation
Damiati, S., Awan, S., Peacock, M., & Schuster, B. (2023) 'Functionalization of Graphene Oxide for Label-Free Electrochemical Detection of Hepatic Cancer Cells', Engineering Proceedings, 35(1). Available at: 10.3390/IECB2023-14599
