ORCID
- Labib, Mahmoud: 0000-0003-4565-2056
Abstract
Nanoneedles are a useful tool for delivering exogenous biomolecules to cells. Although therapeutic applications have been explored, the mechanism regarding how cells interact with nanoneedles remains poorly studied. Here, we present a new approach for the generation of nanoneedles, validated their usefulness in cargo delivery, and studied the underlying genetic modulators during delivery. We fabricated arrays of nanoneedles based on electrodeposition and quantified its efficacy of delivery using fluorescently labeled proteins and siRNAs. Notably, we revealed that our nanoneedles caused the disruption of cell membranes, enhanced the expression of cell–cell junction proteins, and downregulated the expression of transcriptional factors of NFκB pathways. This perturbation trapped most of the cells in G2 phase, in which the cells have the highest endocytosis activities. Taken together, this system provides a new model for the study of interactions between cells and high-aspect-ratio materials.
DOI
10.1021/acs.nolett.2c05083
Publication Date
2023-07-12
Publication Title
Nano Letters
Volume
23
Issue
13
ISSN
1530-6984
Embargo Period
2024-04-10
Organisational Unit
Peninsula Medical School
First Page
5877
Last Page
5885
Recommended Citation
Wang, Z., Wang, H., Lin, S., Labib, M., Ahmed, S., Das, J., Angers, S., Sargent, E., & Kelley, S. (2023) 'Efficient Delivery of Biological Cargos into Primary Cells by Electrodeposited Nanoneedles via Cell-Cycle-Dependent Endocytosis', Nano Letters, 23(13), pp. 5877-5885. Available at: https://doi.org/10.1021/acs.nolett.2c05083
