ORCID
- Jones, Matt: 0000-0003-4723-3277
Abstract
Endosomal Sorting Complex Required for Transport (ESCRT) proteins can be transiently recruited to the plasma membrane for membrane repair and formation of extracellular vesicles. Here, we discovered micrometer-sized worm-shaped ESCRT structures that stably persist for multiple hours at the plasma membrane of macrophages, dendritic cells, and fibroblasts. These structures surround clusters of integrins and known cargoes of extracellular vesicles. The ESCRT structures are tightly connected to the cellular support and are left behind by the cells together with surrounding patches of membrane. The phospholipid composition is altered at the position of the ESCRT structures, and the actin cytoskeleton is locally degraded, which are hallmarks of membrane damage and extracellular vesicle formation. Disruption of actin polymerization increased the formation of the ESCRT structures and cell adhesion. The ESCRT structures were also present at plasma membrane contact sites with membrane-disrupting silica crystals. We propose that the ESCRT proteins are recruited to adhesion-induced membrane tears to induce extracellular shedding of the damaged membrane.
DOI
10.1083/jcb.202205130
Publication Date
2023-03-27
Publication Title
Journal of Cell Biology
Volume
222
Issue
7
ISSN
0021-9525
Embargo Period
2023-08-02
Organisational Unit
Peninsula Medical School
Recommended Citation
Stempels, F. C., Jiang, M., Warner, H., Moser, M., Janssens, M., Maassen, S., Nelen, I., de, B., Jiemy, W., Knight, D., Selley, J., O’cualain, R., Baranov, M., Burgers, T., Sansevrino, R., Milovanovic, D., Heeringa, P., Jones, M., Vlijm, R., ter, B., & van, d. (2023) 'Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters', Journal of Cell Biology, 222(7). Available at: https://doi.org/10.1083/jcb.202205130
