ORCID
- Michael J. Dillon: 0000-0001-6751-7678
Abstract
A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were evaluated for its suitability as a screening method for the detection of toxins in shellfish. The marine toxin-specific antibodies were class-specific, and there was no cross-reactivity between the three toxin groups. The test is capable of detecting all three marine toxin groups, with working ranges of 0.2–1.5, 2.5–65.0, and 8.2–140.3 ng/mL for okadaic acid, saxitoxin, and domoic acid, respectively. This allows the multiplex LFA to detect all three toxin groups at the EU regulatory limits, with a single sample extraction method and dilution volume. No matrix effects were observed on the performance of the LFA with mussel samples spiked with toxins. The developed LFA uses a simple and pocket-sized, portable Cube Reader to provide an accurate result. We also evaluated the use of this Cube Reader with commercially available monoplex lateral flow assays for marine toxins.
DOI Link
Publication Date
2022-09-06
Publication Title
Environmental Science & Technology
Volume
56
Issue
17
ISSN
0013-936X
Acceptance Date
2022-07-29
Deposit Date
2023-01-26
Embargo Period
2023-01-28
First Page
12210
Last Page
12217
Recommended Citation
Mills, C., Dillon, M., Kulabhusan, P., Senovilla-Herrero, D., & Campbell, K. (2022) 'Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins', Environmental Science & Technology, 56(17), pp. 12210-12217. Available at: 10.1021/acs.est.2c02339
