ORCID
- Dillon, Michael: 0000-0001-6751-7678
Abstract
A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were evaluated for its suitability as a screening method for the detection of toxins in shellfish. The marine toxin-specific antibodies were class-specific, and there was no cross-reactivity between the three toxin groups. The test is capable of detecting all three marine toxin groups, with working ranges of 0.2–1.5, 2.5–65.0, and 8.2–140.3 ng/mL for okadaic acid, saxitoxin, and domoic acid, respectively. This allows the multiplex LFA to detect all three toxin groups at the EU regulatory limits, with a single sample extraction method and dilution volume. No matrix effects were observed on the performance of the LFA with mussel samples spiked with toxins. The developed LFA uses a simple and pocket-sized, portable Cube Reader to provide an accurate result. We also evaluated the use of this Cube Reader with commercially available monoplex lateral flow assays for marine toxins.
DOI
10.1021/acs.est.2c02339
Publication Date
2022-09-06
Publication Title
Environmental Science & Technology
Volume
56
Issue
17
ISSN
0013-936X
Embargo Period
2023-01-28
Organisational Unit
Peninsula Medical School
First Page
12210
Last Page
12217
Recommended Citation
Mills, C., Dillon, M., Kulabhusan, P., Senovilla-Herrero, D., & Campbell, K. (2022) 'Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins', Environmental Science & Technology, 56(17), pp. 12210-12217. Available at: https://doi.org/10.1021/acs.est.2c02339
