Abstract
Analysis of extracts from two woad species (Isatis tinctoria and Isatis indigotica) and Polygonum tinctorium revealed that only one indigo precursor (indican) was present in Polygonum, but two precursors were found in Isatis spp. This was done using high performance liquid chromatography (HPLC), coupled to an evaporative light scattering detector (ELSD). In Isatis spp., the indigo precursors indican and a fraction representing isatan B were identified. The proportion of indican and isatan B was different between the two Isatis spp. tested. For the first time, it was possible to quantify the precursors in woad plant species, and the results were found to be in good agreement with those made from total indigo quantification using two different spectrophotometric methods or a derivatization technique.
DOI
10.1021/bp0300624
Publication Date
2004-01-01
Publication Title
Biotechnol Prog
Volume
20
Issue
4
ISSN
8756-7938
Organisational Unit
Peninsula Medical School
Keywords
Chromatography, High Pressure Liquid, Indigo Carmine, Indoles, Isatis, Light, Mass Spectrometry, Plant Leaves, Polygonum, Reference Standards, Scattering, Radiation
First Page
1289
Last Page
1292
Recommended Citation
Gilbert, K., Maule, H., Rudolph, B., Lewis, M., Vandenburg, H., Sales, E., Tozzi, S., & Cooke, D. (2004) 'Quantitative analysis of indigo and indigo precursors in leaves of Isatis spp. and Polygonum tinctorium.', Biotechnol Prog, 20(4), pp. 1289-1292. Available at: https://doi.org/10.1021/bp0300624
