ORCID
- Hanemann, Oliver: 0000-0002-1951-1025
Abstract
The majority of meningiomas are grade I, but some grade I tumours are clinically more aggressive. Recent advances in the genetic study of meningiomas has allowed investigation into the influence of genetics on the tumour microenvironment, which is important for tumorigenesis. We have established that the endpoint genotyping method Kompetitive Allele Specific PCR (KASP™) is a fast, reliable method for the screening of meningioma samples into different non-NF2 mutational groups using a standard real-time PCR instrument. This genotyping method and four-colour flow cytometry has enabled us to assess the variability in the largest immune cell infiltrate population, M2 macrophages (CD45+HLA-DR+CD14+CD163+) in 42 meningioma samples, and to suggest that underlying genetics is relevant. Further immunohistochemistry analysis comparing AKT1 E17K mutants to WHO grade I NF2-negative samples showed significantly lower levels of CD163-positive activated M2 macrophages in meningiomas with mutated AKT1 E17K, signifying a more immunosuppressive tumour microenvironment in NF2 meningiomas. Our data suggested that underlying tumour genetics play a part in the development of the immune composition of the tumour microenvironment. Stratifying meningiomas by mutational status and correlating this with their cellular composition will aid in the development of new immunotherapies for patients.
DOI
10.3390/ijms21041273
Publication Date
2020-02-13
Publication Title
International Journal of Molecular Sciences
Volume
21
Issue
4
First Page
1273
Last Page
1273
ISSN
1661-6596
Embargo Period
2020-07-10
Organisational Unit
Peninsula Medical School
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Adams, C. L., Ercolano, E., Ferluga, S., Sofela, A., Dave, F., Negroni, C., Kurian, K., Hilton, D., & Hanemann, C. (2020) 'A Rapid Robust Method for Subgrouping Non-NF2 Meningiomas According to Genotype and Detection of Lower Levels of M2 Macrophages in AKT1 E17K Mutated Tumours', International Journal of Molecular Sciences, 21(4), pp. 1273-1273. Available at: https://doi.org/10.3390/ijms21041273
