ORCID
- Shouqing Luo: 0000-0002-7998-3059
Abstract
SQSTM1/p62 droplets play crucial roles in droplets-based macroautophagy/autophagy including selective autophagy and bulk autophagy. We observed that under several stress milieus, SQSTM1 droplets entirely colocalize with P-body markers, and these stress-induced SQSTM1 droplets contain mRNAs. We thus determined that under certain stress conditions, autophagic SQSTM1 droplets are converted to a type of enlarged P-bodies, designated SQSTM1/p62-dependent P-bodies (pd-PBs). Stress-enhanced SQSTM1 droplet formation drives the nucleation of pd-PBs through the interaction between SQSTM1 and the RNA-binding protein DDX6. Furthermore, pd-PBs sequester PYCARD, facilitating the assembly of NLRP3 inflammasomes, and in turn induce inflammation-related cytotoxicity. Our study suggests that under stress settings, autophagic SQSTM1 droplets are transformed to pd-PBs, underlining a critical role of SQSTM1 in P-body condensation.
DOI
10.1080/15548627.2024.2340413
Publication Date
2024-01-01
Publication Title
Autophagy
Volume
20
Issue
9
ISSN
1554-8627
Keywords
Autophagy, NLRP3 inflammasome, P-bodies, PYCARD, SQSTM1, Sequestosome-1 Protein/metabolism, Animals, Inflammasomes/metabolism, Autophagy/physiology, Humans, Mice, DEAD-box RNA Helicases/metabolism
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
First Page
2100
Last Page
2101
Recommended Citation
Luo, S., Valionyte, E., Barrow, E., Baxter, C., & Herath, S. (2024) 'Transformation of autophagic SQSTM1 droplets to SQSTM1-dependent P-bodies', Autophagy, 20(9), pp. 2100-2101. Available at: https://doi.org/10.1080/15548627.2024.2340413
