Disruption of Microtubule Integrity Initiates Mitosis during CNS Repair
dc.contributor.author | Bossing, Torsten | |
dc.contributor.author | Barros, Claudia | |
dc.contributor.author | Fischer, B | |
dc.contributor.author | Russell, S | |
dc.contributor.author | Shepherd, D | |
dc.date.accessioned | 2016-10-26T21:29:54Z | |
dc.date.available | 2016-10-26T21:29:54Z | |
dc.date.issued | 2012-08 | |
dc.identifier.issn | 1534-5807 | |
dc.identifier.issn | 1878-1551 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/6644 | |
dc.description.abstract |
Mechanisms of CNS repair have vital medical implications. We show that traumatic injury to the ventral midline of the embryonic Drosophila CNS activates cell divisions to replace lost cells. A pilot screen analyzing transcriptomes of single cells during repair pointed to downregulation of the microtubule-stabilizing GTPase mitochondrial Rho (Miro) and upregulation of the Jun transcription factor Jun-related antigen (Jra). Ectopic Miro expression can prevent midline divisions after damage, whereas Miro depletion destabilizes cortical β-tubulin and increases divisions. Disruption of cortical microtubules, either by chemical depolymerization or by overexpression of monomeric tubulin, triggers ectopic mitosis in the midline and induces Jra expression. Conversely, loss of Jra renders midline cells unable to replace damaged siblings. Our data indicate that upon injury, the integrity of the microtubule cytoskeleton controls cell division in the CNS midline, triggering extra mitosis to replace lost cells. The conservation of the identified molecules suggests that similar mechanisms may operate in vertebrates. | |
dc.format.extent | 433-440 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Elsevier BV | |
dc.subject | Animals | |
dc.subject | Cell Differentiation | |
dc.subject | Drosophila Proteins | |
dc.subject | Drosophila melanogaster | |
dc.subject | Enzyme Activation | |
dc.subject | Microtubules | |
dc.subject | Mitosis | |
dc.subject | Proto-Oncogene Proteins c-jun | |
dc.subject | Tubulin | |
dc.subject | rho GTP-Binding Proteins | |
dc.title | Disruption of Microtubule Integrity Initiates Mitosis during CNS Repair | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000307695500020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 2 | |
plymouth.volume | 23 | |
plymouth.publication-status | Published | |
plymouth.journal | Developmental Cell | |
dc.identifier.doi | 10.1016/j.devcel.2012.06.002 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/Peninsula Medical School | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA03 Allied Health Professions, Dentistry, Nursing and Pharmacy | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2012-06-04 | |
dc.identifier.eissn | 1878-1551 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1016/j.devcel.2012.06.002 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2012-08-14 | |
rioxxterms.type | Journal Article/Review | |
plymouth.oa-location | http://www.cell.com/developmental-cell/pdf/S1534-5807(12)00275-4.pdf |