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dc.contributor.authorStanden, BTen
dc.contributor.authorRodiles, Aen
dc.contributor.authorPeggs, DLen
dc.contributor.authorDavies, SJen
dc.contributor.authorSantos, GAen
dc.contributor.authorMerrifield, DLen
dc.date.accessioned2015-08-13T21:55:25Z
dc.date.available2015-08-13T21:55:25Z
dc.date.issued2015-10en
dc.identifier.urihttp://hdl.handle.net/10026.1/3521
dc.description.abstract

The intestinal microbiota and morphology of tilapia (Oreochromis niloticus) were investigated after the application of a multi-species probiotic containing Lactobacillus reuteri, Bacillus subtilis, Enterococcus faecium and Pediococcus acidilactici (AquaStar(®) Growout). Tilapia (55.03 ± 0.44 g) were fed either a control diet or a probiotic diet (control diet supplemented with AquaStar(®) Growout at 5 g kg(-1)). After four and eight weeks, culture-dependent analysis showed higher levels of lactic acid bacteria (LAB), enterococci and Bacillus spp. in the mucosa and digesta of fish fed AquaStar(®) Growout. At week four, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) revealed a higher similarity within the probiotic fed replicates than replicates of the control group; after eight weeks, the compositional dissimilarity of the microbiome profiles between the groups was greater than the dissimilarities within each group (P < 0.05). High-throughput sequencing revealed that the probiotic treatment significantly reduced the number of operational taxonomic units and species richness in the digesta. Significantly higher proportions of reads belonging to Proteobacteria and Cyanobacteria were detected in the control group whereas the probiotic-fed fish displayed a significantly higher abundance of reads assigned to the Firmicutes (which accounted for >99 % of reads). Bacillus, Cetobacterium and Mycobacterium were the dominant genera in the digesta of control fish whereas Bacillus, Enterococcus and Pediococcus were the largest constituents in probiotic-fed fish. The addition of AquaStar(®) Growout to tilapia diets led to increased populations of intraepithelial leucocytes, a higher absorptive surface area index and higher microvilli density in the intestine. These data suggest that AquaStar(®) Growout can modulate both the intestinal microbiota and morphology of tilapia.

en
dc.format.extent8403 - 8417en
dc.languageengen
dc.language.isoengen
dc.subjectHigh-throughput sequencingen
dc.subjectIntestinal microbiotaen
dc.subjectIntraepithelial leucocyte (IEL)en
dc.subjectMicroscopyen
dc.subjectProbioticen
dc.subjectTilapiaen
dc.subjectAnimalsen
dc.subjectCichlidsen
dc.subjectGastrointestinal Microbiomeen
dc.subjectGastrointestinal Tracten
dc.subjectHigh-Throughput Nucleotide Sequencingen
dc.subjectHistocytochemistryen
dc.subjectMicroscopy, Electron, Transmissionen
dc.subjectProbioticsen
dc.titleModulation of the intestinal microbiota and morphology of tilapia, Oreochromis niloticus, following the application of a multi-species probiotic.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/26115752en
plymouth.issue20en
plymouth.volume99en
plymouth.publication-statusPublisheden
plymouth.journalAppl Microbiol Biotechnolen
dc.identifier.doi10.1007/s00253-015-6702-2en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
dc.publisher.placeGermanyen
dcterms.dateAccepted2015-05-17en
dc.identifier.eissn1432-0614en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1007/s00253-015-6702-2en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-10en
rioxxterms.typeJournal Article/Reviewen
plymouth.oa-locationhttp://link.springer.com/article/10.1007/s00253-015-6702-2?no-access=trueen


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