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dc.contributor.supervisorSimon, K. Jackson
dc.contributor.authorSattar, Anas Akram
dc.contributor.otherPeninsula Schools of Medicine and Dentistryen_US
dc.date.accessioned2015-01-05T09:19:57Z
dc.date.available2015-01-05T09:19:57Z
dc.date.issued2014
dc.identifier10250431en_US
dc.identifier.urihttp://hdl.handle.net/10026.1/3185
dc.description.abstract

The quality of marine bathing water is currently assessed by monitoring the levels of faecal indicator bacteria. Among other drawbacks, results are retrospective using the traditional culture based methods. A rapid method is thus needed as an early warning to bathers for bacterial contamination in marine bathing waters. Total lipopolysaccharide (LPS) was chosen here as a potential general biomarker for bacterial contamination. Levels of total LPS, measured using a Kinetic QCL™ Limulus Amebocyte Lysate (LAL) assay, highly correlated with enumerated Escherichia coli and Bacteroides species. Levels of LPS in excess of 50 EU mL-1 were found to equate with water that was unsuitable for bathing under the current European Union regulations. Results showed that monitoring the levels of total LPS has a potential applicability as a rapid method for screening the quality of marine bathing water. More importantly, the LAL assay overcome the retrospective results when using culture based assessment since the LAL assay takes less than 30 minutes. Although false positive events were not detected, the occurrence of a false positive has been hypothesised, hence a more specific faecal biomarker was also investigated. LPS of five Bacteroides species (B. fragilis, B. caccae, B. ovatus, B. xylanisolvens and B. finegoldii) isolated from marine bathing waters samples were successfully profiled and showed high similarity between isolates in LPS gel electrophoresis banding pattern. Similar results were shown when investigating the endotoxic activity of Bacteroides species with the Kinetic QCL™ LAL assay. The potential biological relevance of Bacteroides LPS was also investigated in cell culture models indicating that Bacteroides showed similar induction of proinflammatory cytokines (TNF-α, IL-6 and IL-1α) and generally the biological activity was approximately 100 fold less than E. coli LPS. In addition, an ELISA assay was designed for the detection of Bacteroides LPS. Results showed that the Bacteroides LPS has a high potential to be used as a faecal biomarker, however, further work is required to develop a fully functional assay. The potential biological relevance of LPS present in contaminated bathing waters was also investigated in cell culture models. Results showed that there is a significant difference in the production of proinflammatory cytokines in comparison to “clean” bathing waters. Thus, results suggest that the European Directive regulations should be extended to cover the levels of total LPS in bathing waters to assure safety to the users of marine recreational water.

en_US
dc.language.isoenen_US
dc.publisherPlymouth Universityen_US
dc.subjectEndotoxinen_US
dc.subjectHuman healthen_US
dc.subjectLipopolysaccharideen_US
dc.subjectRapid assayen_US
dc.subjectBacteriaen_US
dc.subjectMolecular biologyen_US
dc.subjectBathing seawateren_US
dc.subjectcytokinesen_US
dc.subjectBacteroidesen_US
dc.subjectEscherichia colien_US
dc.subjectEnterococcien_US
dc.subjectTotal coliformen_US
dc.subjectCell Cultureen_US
dc.subjectMonocyteen_US
dc.subjectMacrophageen_US
dc.subjectMPIen_US
dc.subjectMM6en_US
dc.subjectBiomedical sciencesen_US
dc.titleLipopolysaccharide in marine bathing water; a potential real-time biomarker of bacterial contamination and relevance to human healthen_US
dc.typeDoctorateen_US
plymouth.versionFull versionen_US


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