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dc.contributor.authorJha, A
dc.date.accessioned2023-05-04T11:21:26Z
dc.date.available2023-05-04T11:21:26Z
dc.date.issued2023-03-01
dc.identifier.issn1754-2189
dc.identifier.issn1750-2799
dc.identifier.urihttps://pearl.plymouth.ac.uk/handle/10026.1/20786
dc.descriptionFile replaced (docx to pdf) on 5.5.23 by NK (LDS)
dc.description.abstract

The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA–DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.

dc.format.extent929-989
dc.format.mediumPrint-Electronic
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectAnimals
dc.subjectHumans
dc.subjectComet Assay
dc.subjectDNA Damage
dc.subjectPyrimidine Dimers
dc.subjectEukaryotic Cells
dc.subjectDNA
dc.titleMeasuring DNA modifications with the comet assay: a compendium of protocols
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36707722
plymouth.issue3
plymouth.volume18
plymouth.publisher-urlhttp://dx.doi.org/10.1038/s41596-022-00754-y
plymouth.publication-statusPublished
plymouth.journalNATURE PROTOCOLS | www.nature.com/nprot
dc.identifier.doi10.1038/s41596-022-00754-y
plymouth.organisational-group|Plymouth
plymouth.organisational-group|Plymouth|Research Groups
plymouth.organisational-group|Plymouth|Faculty of Science and Engineering
plymouth.organisational-group|Plymouth|Faculty of Science and Engineering|School of Biological and Marine Sciences
plymouth.organisational-group|Plymouth|Research Groups|Marine Institute
plymouth.organisational-group|Plymouth|REF 2021 Researchers by UoA
plymouth.organisational-group|Plymouth|Users by role
plymouth.organisational-group|Plymouth|Users by role|Academics
plymouth.organisational-group|Plymouth|REF 2021 Researchers by UoA|UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group|Plymouth|Admin Group - REF
plymouth.organisational-group|Plymouth|Admin Group - REF|REF Admin Group - FoSE
plymouth.organisational-group|Plymouth|Users by role|Researchers in ResearchFish submission
dc.publisher.placeEngland
dcterms.dateAccepted2022-07-05
dc.date.updated2023-05-04T11:21:26Z
dc.rights.embargodate2023-7-27
dc.identifier.eissn1750-2799
dc.rights.embargoperiodforever
rioxxterms.versionofrecord10.1038/s41596-022-00754-y


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