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dc.contributor.authorBonthond, G
dc.contributor.authorBarilo, A
dc.contributor.authorAllen, RJ
dc.contributor.authorCunliffe, M
dc.contributor.authorKrueger‐Hadfield, SA
dc.contributor.editorLane C
dc.date.accessioned2022-03-07T11:34:28Z
dc.date.available2022-03-07T11:34:28Z
dc.date.issued2022-03-04
dc.identifier.issn0022-3646
dc.identifier.issn1529-8817
dc.identifier.urihttp://hdl.handle.net/10026.1/18901
dc.description.abstract

<jats:p>Fungal symbionts of terrestrial plants are among the most widespread and well‐studied symbioses, relatively little is known about fungi that are associated with macroalgae. To fill the gap in marine fungal taxonomy, we combined simple culture methods with amplicon sequencing to characterize the fungal communities associated with three brown (<jats:italic>Sargassum muticum</jats:italic>, <jats:italic>Pelvetia canaliculata</jats:italic>, and <jats:italic>Himanthalia elongata</jats:italic>) and two red (<jats:italic>Mastocarpus stellatus</jats:italic> and <jats:italic>Chondrus crispus</jats:italic>) macroalgae from one intertidal zone. In addition to characterizing novel fungal diversity, we tested three hypotheses: fungal diversity and community composition vary (i) among species distributed at different tidal heights, (ii) among tissue types (apices, mid‐thallus, and stipe), and (iii) among “isomorphic” <jats:italic>C. crispus</jats:italic> life cycle stages. Almost 70% of our reads were classified as Ascomycota, 29% as Basidiomycota, and 1% that could not be classified to a phylum. Thirty fungal isolates were obtained, 18 of which were also detected with amplicon sequencing. Fungal communities differed by host and tissue type. Interestingly, <jats:italic>P</jats:italic>. <jats:italic>canaliculata</jats:italic>, a fucoid at the extreme high intertidal, did not show differences in fungal diversity across the thallus. As found in filamentous algal endophytes, fungal diversity varied among the three life cycle stages in <jats:italic>C</jats:italic>. <jats:italic>crispus</jats:italic>. Female gametophytes were also compositionally more dispersed as compared to the fewer variable tetrasporophytes and male gametophytes. We demonstrate the utility of combining relatively simple cultivation and sequencing approaches to characterize and study macroalgal–fungal associations and highlight the need to understand the role of fungi in near‐shore marine ecosystems.</jats:p>

dc.format.extent330-342
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherWiley
dc.subjectevolutionary ecology
dc.subjectfungi
dc.subjectholobiont
dc.subjectlife cycle
dc.subjectmarine
dc.subjectmycology
dc.subjectseaweed
dc.subjectsymbiosis
dc.titleFungal endophytes vary by species, tissue type, and life cycle stage in intertidal macroalgae
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35090190
plymouth.issue2
plymouth.volume58
plymouth.publisher-urlhttp://dx.doi.org/10.1111/jpy.13237
plymouth.publication-statusPublished
plymouth.journalJournal of Phycology: an international journal of algal research
dc.identifier.doi10.1111/jpy.13237
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA07 Earth Systems and Environmental Sciences
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2022-01-04
dc.rights.embargodate2022-3-8
dc.identifier.eissn1529-8817
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1111/jpy.13237
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2022-03-04
rioxxterms.typeJournal Article/Review


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