Diurnal, Circadian, and Photomorphogenic Analyses in Magnaporthe oryzae

Abstract

Circadian rhythms have been shown to play an important role in plant-pathogen interactions between plants and fungi. These protocols describe the methodology used to determine the function and characteristics of the diurnal and circadian behavior in the hemibiotrophic fungal pathogen, Magnaporthe oryzae. Here we describe methods to determine how conidiation, conidial development, and pathogenicity may be altered in M. oryzae as a result of differing diurnal or circadian environmental conditions.