Active bacterioplankton community response to dissolved ‘free’ deoxyribonucleic acid (dDNA) in surface coastal marine waters
dc.contributor.author | Taylor, JD | |
dc.contributor.author | Bird, KE | |
dc.contributor.author | Widdicome, CE | |
dc.contributor.author | Cunliffe, Michael | |
dc.date.accessioned | 2020-07-16T07:55:35Z | |
dc.date.issued | 2018-10-01 | |
dc.identifier.issn | 0168-6496 | |
dc.identifier.issn | 1574-6941 | |
dc.identifier.other | ARTN fiy132 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/16043 | |
dc.description.abstract |
Seawater contains dissolved 'free' DNA (dDNA) that is part of a larger <0.2 µm pool of DNA (D-DNA) including viruses and uncharacterised bound DNA. Previous studies have shown that bacterioplankton readily degrade dDNA, and culture-based approaches have identified several potential dDNA-utilising taxa. This study characterised the seasonal variation in D-DNA concentrations at Station L4, a coastal marine observatory in the Western English Channel, and linked changes in concentration to cognate physicochemical and biological factors. The impact of dDNA addition on active bacterioplankton communities at Station L4 was then determined using 16S rRNA high-throughput sequencing and RNA Stable Isotope Probing (RNA SIP) with 13C-labelled diatom-derived dDNA. Compared to other major bacterioplankton orders, the Rhodobacterales actively responded to dDNA additions in amended microcosms and RNA SIP identified two Rhodobacterales populations most closely associated with the genera Halocynthiibacter and Sulfitobacter that assimilated the 13C-labelled dDNA. Here we demonstrate that dDNA is a source of dissolved organic carbon for some members of the major bacterioplankton group the Marine Roseobacter Clade. This study enhances our understanding of roles of specific bacterioplankton taxa in dissolved organic matter cycling in coastal waters with potential implications for nitrogen and phosphorus regeneration processes. | |
dc.format.medium | ||
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Oxford University Press (OUP) | |
dc.subject | DNA | |
dc.subject | bacterioplankton | |
dc.subject | stable-isotope probing | |
dc.subject | DON | |
dc.subject | DOC | |
dc.subject | DOP | |
dc.title | Active bacterioplankton community response to dissolved ‘free’ deoxyribonucleic acid (dDNA) in surface coastal marine waters | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000448167900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 10 | |
plymouth.volume | 94 | |
plymouth.publication-status | Published | |
plymouth.journal | FEMS Microbiology Ecology | |
dc.identifier.doi | 10.1093/femsec/fiy132 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA07 Earth Systems and Environmental Sciences | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | England | |
dcterms.dateAccepted | 2018-07-10 | |
dc.rights.embargodate | 2020-7-18 | |
dc.identifier.eissn | 1574-6941 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.funder | Natural Environment Research Council | |
rioxxterms.identifier.project | Development and application of eDNA tools to assess the structure and function of coastal sea ecosystems (MARINe-DNA) | |
rioxxterms.versionofrecord | 10.1093/femsec/fiy132 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2018-10-01 | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | Development and application of eDNA tools to assess the structure and function of coastal sea ecosystems (MARINe-DNA)::Natural Environment Research Council |