Identification and expression analysis of CBF/DREB1 and COR15 genes in mutants of Brassica oleracea var. botrytis with enhanced proline production and frost resistance.
MetadataShow full item record
Frost resistant mutants of Brassica oleracea var. botrytis were investigated for the presence of CBF/DREB1 and COR15a gene products and induced frost resistance. Total RNA of clones was isolated after 3 h, 6 h, 24 h and 14 d acclimation at 4 °C and proteins and free proline were isolated after 14 d acclimation. cDNA was produced using RT-PCR and the first CBF gene in B. oleracea detected and did quantify. Through SDS-PAGE and Western blotting, the COR15a protein was detected for the first time in B. oleracea. The results confirmed the first report of the presence of BoCBF/DREB1 in B. oleracea and this only appeared under cold acclimation. The sequence analysis of predicted amino acids revealed a very high homology (90%) with CBF sequences of other Brassica species (BnCBF5/DREB1, BrDREB1 and BjDREB1B) and homology reduced to 67% when compared to plants other than Brassicas. BoCBF/DREB1 transcript levels increased up to 24 h acclimation and then declined. Some mutants showed BoCBF/DREB1 expression at 3 h while others only after 6 h and 24 h acclimation. The genotypes showed positive significant correlation between BoCBF/DREB1 expression and frost resistance (R(2) = 0.9343). The proline level under acclimation increased about 8 fold and demonstrated positive and significant correlation with BoCBF/DREB1 expression. Proline also showed positive and significant correlation with frost resistance under cold acclimation but very not under non-acclimation. All clones were positive for COR15a protein after 14 d cold acclimation and expression correlated with frost resistance. Under non-acclimation COR15a was constitutively expressed in 3 mutants.
Place of Publication
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Stevens, K; Weynberg, K; Bellas, C; Brown, S; Brownlee, C; Brown, MT; Schroeder, DC (United StatesUnited StatesUnited StatesUnited StatesUnited States, 2014)Phaeoviruses infect the brown algae, which are major contributors to primary production of coastal waters and estuaries. They exploit a Persistent evolutionary strategy akin to a K- selected life strategy via genome ...
Petty, NK; Ben Zakour, NL; Stanton-Cook, M; Skippington, E; Totsika, M; Forde, BM; Phan, M-D; Gomes Moriel, D; Peters, KM; Davies, M; Rogers, BA; Dougan, G; Rodriguez-Baño, J; Pascual, A; Pitout, JDD; Upton, M; Paterson, DL; Walsh, TR; Schembri, MA; Beatson, SA (United StatesUnited StatesUnited StatesUnited StatesUnited States, 2014-04-15)Escherichia coli sequence type 131 (ST131) is a globally disseminated, multidrug resistant (MDR) clone responsible for a high proportion of urinary tract and bloodstream infections. The rapid emergence and successful ...
Micropalaeontology, Palaeoenvironments and Sequence Stratigraphy of the Sulaiy Formation of Eastern Saudi Arabia ALENEZI, SALEH (Plymouth University, 2016)The Sulaiy Formation, which is the oldest unit in the Lower Cretaceous succession, is conformably overlain by the Yamama Formation and it is a challenge to identify the precise age of the two formations using foraminifera ...