The minimum inhibitory concentration (MIC) assay with Escherichia coli: an early tier in the environmental hazard assessment of nanomaterials?
dc.contributor.author | Vassallo, J | |
dc.contributor.author | Besinis, Alexandros | |
dc.contributor.author | Boden, Rich | |
dc.contributor.author | Handy, Richard | |
dc.date.accessioned | 2018-09-13T07:37:50Z | |
dc.date.issued | 2018-10-30 | |
dc.identifier.issn | 0147-6513 | |
dc.identifier.issn | 1090-2414 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/12346 | |
dc.description.abstract |
There are now over a thousand nano-containing products on the market and the antibacterial properties of some nanomaterials has created interest in their use as cleaning agents, biocides and disinfectants. Engineered nanomaterials (ENMs) are being released into the environment and this raises concerns about their effects on microbes in the receiving ecosystems. This study evaluated the bacterial toxicity of a wide range of nanomaterials with different surface coatings on Escherichia coli K-12 MG1655. The minimum inhibitory concentration (MIC) assay, which quantifies the threshold for growth inhibition in suspensions of bacteria, was used to rank the toxicity of silver (Ag), cupric oxide (CuO), cadmium telluride (CdTe) quantum dots, titanium dioxide (TiO2), nanodiamonds and multi-walled carbon nanotubes (MWCNTs). Bacteria were exposed for 12 h at 37 °C to a dilution series of the test suspensions in 96-well plates. The precision and accuracy of the method was good with coefficients of variation < 10%. In terms of the measured MIC values, the toxicity order of the ENMs was as follows: CdTe quantum dots ammonium-coated, 6 mg L-1 > Ag nanoparticles, 12 mg L-1 > CdTe quantum dots carboxylate-coated, 25 mg L-1 > CdTe quantum dots polyethylene glycol-coated, 100 mg L-1. The MIC values were above the highest test concentration used (100 mg L-1) for CuO, TiO2, nanodiamonds and MWCNTs, indicating low toxicity. The MIC assay can be a useful tool for the initial steps of ENMs hazard assessment. | |
dc.format.extent | 633-646 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.subject | Bioassay | |
dc.subject | Ranking test | |
dc.subject | Nanotechnology | |
dc.subject | Tellurium | |
dc.subject | Antimicrobial | |
dc.subject | Metal dissolution | |
dc.title | The minimum inhibitory concentration (MIC) assay with Escherichia coli: an early tier in the environmental hazard assessment of nanomaterials? | |
dc.type | journal-article | |
dc.type | Journal Article | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000440879500073&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.volume | 162 | |
plymouth.publication-status | Published | |
plymouth.journal | Ecotoxicology and Environmental Safety | |
dc.identifier.doi | 10.1016/j.ecoenv.2018.06.085 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Engineering, Computing and Mathematics | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA12 Engineering | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Marine Institute | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | Netherlands | |
dcterms.dateAccepted | 2018-06-27 | |
dc.rights.embargodate | 2019-7-19 | |
dc.identifier.eissn | 1090-2414 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1016/j.ecoenv.2018.06.085 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2018-10-30 | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | NANOSOLUTIONS Biological Foundation for the Safety Classification of Engineered Nanomaterials (ENM): Systems Biology Approaches to Understand::European Commission FP7 |