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dc.contributor.authorLiu, Ben
dc.contributor.authorCampo, EMen
dc.contributor.authorBossing, Ten
dc.date.accessioned2018-08-13T14:24:23Z
dc.date.available2018-08-13T14:24:23Z
dc.date.issued2014en
dc.identifier.urihttp://hdl.handle.net/10026.1/12097
dc.description.abstract

Different toxicity tests for carbon nanotubes (CNT) have been developed to assess their impact on human health and on aquatic and terrestrial animal and plant life. We present a new model, the fruit fly Drosophila embryo offering the opportunity for rapid, inexpensive and detailed analysis of CNTs toxicity during embryonic development. We show that injected DiI labelled multi-walled carbon nanotubes (MWCNTs) become incorporated into cells in early Drosophila embryos, allowing the study of the consequences of cellular uptake of CNTs on cell communication, tissue and organ formation in living embryos. Fluorescently labelled subcellular structures showed that MWCNTs remained cytoplasmic and were excluded from the nucleus. Analysis of developing ectodermal and neural stem cells in MWCNTs injected embryos revealed normal division patterns and differentiation capacity. However, an increase in cell death of ectodermal but not of neural stem cells was observed, indicating stem cell-specific vulnerability to MWCNT exposure. The ease of CNT embryo injections, the possibility of detailed morphological and genomic analysis and the low costs make Drosophila embryos a system of choice to assess potential developmental and cellular effects of CNTs and test their use in future CNT based new therapies including drug delivery.

en
dc.format.extente88681 - ?en
dc.languageengen
dc.language.isoengen
dc.subjectAnimalsen
dc.subjectBiological Transporten
dc.subjectCell Deathen
dc.subjectDrosophila melanogasteren
dc.subjectEctodermen
dc.subjectEmbryo, Nonmammalianen
dc.subjectEmbryonic Stem Cellsen
dc.subjectNanotubes, Carbonen
dc.subjectToxicity Testsen
dc.titleDrosophila embryos as model to assess cellular and developmental toxicity of multi-walled carbon nanotubes (MWCNT) in living organisms.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/24558411en
plymouth.issue2en
plymouth.volume9en
plymouth.publication-statusPublished onlineen
plymouth.journalPLoS Oneen
dc.identifier.doi10.1371/journal.pone.0088681en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA03 Allied Health Professions, Dentistry, Nursing and Pharmacy
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited Statesen
dcterms.dateAccepted2014-01-09en
dc.identifier.eissn1932-6203en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1371/journal.pone.0088681en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014en
rioxxterms.typeJournal Article/Reviewen


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