Primary structure and sexual stage-specific expression of a LAMMER protein kinase of Plasmodium falciparum.
dc.contributor.author | Li, JL | en |
dc.contributor.author | Targett, GA | en |
dc.contributor.author | Baker, DA | en |
dc.date.accessioned | 2017-11-27T18:41:02Z | |
dc.date.available | 2017-11-27T18:41:02Z | |
dc.date.issued | 2001-04 | en |
dc.identifier.issn | 0020-7519 | en |
dc.identifier.uri | http://hdl.handle.net/10026.1/10323 | |
dc.description.abstract |
We have isolated a LAMMER-like gene from Plasmodium falciparum by vectorette technique. The gene consists of 3316 bp encoding a protein 881 amino acids with a predicted molecular mass of approximately 106.7 kDa. The encoded protein, termed PfLAMMER, is composed of two distinct domains. The N-terminal domain is not related to any previously described protein kinases and has several interesting features including multiple consensus phosphorylation sites for a range of protein kinases, a number of RS/SR dipeptides, a large proportion of charged amino acids, two putative nuclear localisation signals and 14 copies of a tetramer DKYD repeats. The C-terminal domain is characteristic of a kinase in the LAMMER family with the highest homology to the Arabidopsis thaliana AFC3 kinase. Genomic restriction analysis showed that PfLAMMER is encoded by a single copy gene in the parasite genome. A single transcript of approximately 3800 nucleotides is expressed specifically in the sexual stage, indicating that PfLAMMER may be important in regulating the processes of sexual differentiation of the parasite. | en |
dc.format.extent | 387 - 392 | en |
dc.language | eng | en |
dc.language.iso | eng | en |
dc.subject | Amino Acid Sequence | en |
dc.subject | Animals | en |
dc.subject | Base Sequence | en |
dc.subject | Blotting, Northern | en |
dc.subject | Blotting, Southern | en |
dc.subject | Cloning, Molecular | en |
dc.subject | DNA, Protozoan | en |
dc.subject | Gene Expression Regulation, Enzymologic | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Plasmodium falciparum | en |
dc.subject | Polymerase Chain Reaction | en |
dc.subject | Protein Kinases | en |
dc.subject | Protein Serine-Threonine Kinases | en |
dc.subject | RNA, Messenger | en |
dc.subject | Restriction Mapping | en |
dc.subject | Sequence Alignment | en |
dc.subject | Sequence Analysis, DNA | en |
dc.subject | Sex Factors | en |
dc.title | Primary structure and sexual stage-specific expression of a LAMMER protein kinase of Plasmodium falciparum. | en |
dc.type | Journal Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/11306117 | en |
plymouth.issue | 4 | en |
plymouth.volume | 31 | en |
plymouth.publication-status | Published | en |
plymouth.journal | Int J Parasitol | en |
dc.identifier.doi | 10.1016/s0020-7519(01)00126-6 | en |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
dc.publisher.place | England | en |
dc.rights.embargoperiod | Not known | en |
rioxxterms.versionofrecord | 10.1016/s0020-7519(01)00126-6 | en |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | en |
rioxxterms.type | Journal Article/Review | en |