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dc.contributor.authorStubbs, Men
dc.contributor.authorBiswas, Sen
dc.contributor.authorTroy, Hen
dc.contributor.authorLeek, Ren
dc.contributor.authorChung, YLen
dc.contributor.authorLi, JLen
dc.contributor.authorRaval, RRen
dc.contributor.authorTurley, Hen
dc.contributor.authorGatter, Ken
dc.contributor.authorPezzella, Fen
dc.contributor.authorGriffiths, JRen
dc.contributor.authorHarris, ALen

In cultured clear-cell renal carcinoma (CCRCC) 786-0 cells transfected with HIF1 (HIF-1+), HIF-2 (HIF-2+), or empty vector (EV), no significant differences were observed in the growth rates in vitro, but when grown in vivo as xenografts HIF-2 significantly increased, and HIF-1 significantly decreased growth rates, compared to EV tumors. Factors associated with proliferation were increased and factors associated with cell death were decreased in HIF-2+ tumors. Metabolite profiles showed higher glucose and lower lactate and alanine levels in the HIF-2+ tumors whilst immunostaining demonstrated higher pyruvate dehydrogenase and lower pyruvate dehydrogenase kinase 1, compared to control tumors. Taken together, these results suggest that overexpression of HIF-2 in CCRCC 786-0 tumors regulated growth both by maintaining a low level of glycolysis and by allowing more mitochondrial metabolism and tolerance to ROS induced DNA damage. The growth profiles observed may be mediated by adaptive changes to a more oxidative phenotype. Copyright © 2010 Swethajit Biswas et al.

dc.titleEffects of HIF-1 and HIF2 on growth and metabolism of clear-cell renal cell carcinoma 786-0 xenograftsen
dc.typeJournal Article
plymouth.journalJournal of Oncologyen
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Biomedical Research Group/RC Reporting Group BRG
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/Collaboration for the Advancement of Medical Education Research Assessment
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
dc.rights.embargoperiodNot knownen
rioxxterms.typeJournal Article/Reviewen

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