Effects of HIF-1 and HIF2 on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts
dc.contributor.author | Biswas, S | |
dc.contributor.author | Troy, H | |
dc.contributor.author | Leek, R | |
dc.contributor.author | Chung, Y-L | |
dc.contributor.author | Li, Ji-Liang | |
dc.contributor.author | Raval, RR | |
dc.contributor.author | Turley, H | |
dc.contributor.author | Gatter, K | |
dc.contributor.author | Pezzella, F | |
dc.contributor.author | Griffiths, JR | |
dc.contributor.author | Stubbs, M | |
dc.contributor.author | Harris, AL | |
dc.date.accessioned | 2017-11-27T16:45:22Z | |
dc.date.available | 2017-11-27T16:45:22Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 1687-8450 | |
dc.identifier.issn | 1687-8469 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/10311 | |
dc.description.abstract |
<jats:p>In cultured clear-cell renal carcinoma (CCRCC) 786-0 cells transfected with HIF1 (HIF-1+), HIF-2 (HIF-2+), or empty vector (EV), no significant differences were observed in the growth rates<jats:italic>in vitro</jats:italic>, but when grown<jats:italic>in vivo</jats:italic>as xenografts HIF-2 significantly increased, and HIF-1 significantly decreased growth rates, compared to EV tumors. Factors associated with proliferation were increased and factors associated with cell death were decreased in HIF-2+ tumors. Metabolite profiles showed higher glucose and lower lactate and alanine levels in the HIF-2+ tumors whilst immunostaining demonstrated higher pyruvate dehydrogenase and lower pyruvate dehydrogenase kinase 1, compared to control tumors. Taken together, these results suggest that overexpression of HIF-2 in CCRCC 786-0 tumors regulated growth both by maintaining a low level of glycolysis and by allowing more mitochondrial metabolism and tolerance to ROS induced DNA damage. The growth profiles observed may be mediated by adaptive changes to a more oxidative phenotype.</jats:p> | |
dc.format.extent | 1-14 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Hindawi Limited | |
dc.subject | Rare Diseases | |
dc.subject | Cancer | |
dc.subject | Kidney Disease | |
dc.subject | Biotechnology | |
dc.title | Effects of HIF-1 and HIF2 on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts | |
dc.type | journal-article | |
dc.type | Journal Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/20652061 | |
plymouth.volume | 2010 | |
plymouth.publication-status | Published | |
plymouth.journal | Journal of Oncology | |
dc.identifier.doi | 10.1155/2010/757908 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
dc.publisher.place | Egypt | |
dcterms.dateAccepted | 2010-04-22 | |
dc.identifier.eissn | 1687-8469 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1155/2010/757908 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2010 | |
rioxxterms.type | Journal Article/Review |