ORCID

Abstract

The cellular homeostatic response (CHR) and cellular stress response (CSR) work together to maintain homeostasis. Studying phylogenetically closely-related species inhabiting different environments can help investigate the interplay between the CHR and CSR. We conducted reciprocal in situ transplant experiments in a natural CO2 vent (Ischia, Italy), using the sibling annelid species Platynereis cf.. dumerilii and Platynereis cf.. massiliensis which have been shown to have different preferential distributions around the CO2 vent. Following transplantations, we characterised the response of each individual’s proteome, metabolome, and lipidome, to short or long-term exposure to different pCO2 regimes (i.e., high and low), and confirmed its genetic identity. Here we show that different components of the CHR and CSR are utilised at different rates when Platynereis spp. are exposed to different pCO2 regimes, with cellular responses shown to be conserved across species. Metabolome and lipidome responses were dependent on regime of origin, and changed relatively slowly, whereas proteome responses were dependent on transplant type and changed more rapidly. Our results provide new insights to improve our understanding of the interplay between different cellular physiological responses involved in defining the functional phenotype of marine species, and their ability to acclimatise to future projected high pCO2 conditions.

Publication Date

2025-09-29

Publication Title

Scientific Reports

Volume

15

Issue

1

ISSN

2045-2322

Acceptance Date

2025-09-04

Deposit Date

2025-10-16

Funding

We acknowledge the support of the following funding: UKOARP NERC (NE/H017127/1) and University of Plymouth to PC, ASSEMBLE (MetabolAdapt) to PC and LMT, Stazione Zoologica PhD fellowship to ER and the PON-MODO Project (Campania Region) to AMG. At the time of this study MCG was senior investigator at the Ischia Marine Centre (SZN, Naples). DM is supported by FCT I.P. through Scientific Employment Stimulus – Individual Call 2022 (2022.00153.CEECIND; https://doi.org/10.54499/2022.00153.CEECIND/CP1720/CT0016 ). DM also acknowledges FCT I.P. for the financial support to CESAM (UIDP/50017/2020 + UIDB/50017/2020 + LA/P/0094/2020). PC is supported by a NSERC Discovery grant RGPIN-2020-05627. PC is an active member of the inter-institutional strategic research networks Québec-Océan and Resources Aquatiques Québec.

Keywords

Cellular stress response (CSR), Lipidomics, Metabolomics, Ocean acidification, Platynereis, Proteomics

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