ORCID

Abstract

In the present study the impact on gut microbiology and indigenous gut histology of Atlantic salmon (Salmo salar L.) was investigated following feeding of a control and a prebiotic (EWOS prebiosal®) diet and ex vivo exposure to saline or the probiotic bacterium Carnobacterium divergens. The results showed that ex vivo exposure of C. divergens at 108 CFU ml-1 did not cause cell damage to the intestine tract of Atlantic salmon. Furthermore, prior provision of dietary prebiotic elevated the ability of C. divergens to adhere to the epithelium or mucus layer in the proximal intestine, where culturable heterotrophic bacterial levels (which were identified as C. divergens) were elevated by 234% compared to the control. This effect was not apparent in the distal intestine. The ability of isolated carnobacteria from the ex vivo experiment to inhibit growth of two fish pathogenic bacteria (Yersinia rückeri and Aeromonas salmonicida ssp. salmonicida) was assessed. Extra cellular products from all 11 of the isolated carnobacteria strains, plus the type strain Carnobacterium inhibens CCUG 31728, inhibited the in vitro growth of Y. rückeri. However, only extra cellular products from C. divergens isolate 57 inhibited the growth of A. salmonicida ssp. salmonicida.

Publication Date

2011-10-25

Publication Title

Journal of Aquaculture Research and Development

Issue

SPEC. ISSUE 1

ISSN

2155-9546

Keywords

16S rRNA, Carnobacteria, Electron microscopy, Pathogen, Synbiotic

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10.4172/2155-9546.S1-009" data-hide-no-mentions="true">

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