Abstract
Cauliflower is described as a recalcitrant plant to the genetic transformation process from Agrobacterium. Cauliflower curd meristems can be used to produce shoots to clone the mother plants in in-vitro culture. Micro propagation techniques were used in this study at different stages to produce cauliflower explants. The healthy growing explants were transformed with the APX gene using a standard transfection protocol mediated by Agrobacterium. The plants that successfully grew under abiotic stress and presenting a normal plant phenotypic feature were considered transformants. The transfection conditions were optimised during this study and 105Agrobacterium cell/5 plants/pot successfully produced 1 transformed plant/pot.
Publication Date
2012-10-01
Publication Title
Acta Horticulturae
Volume
961
Publisher
International Society of Horticultural Science
Embargo Period
2024-11-22
First Page
167
Last Page
174
Recommended Citation
Al-Swedi, F., Lane, S., & Fuller, M. (2012) 'An improved micro propagation system for successful transformation of cauliflower (Brassica oleracea var. botrytis).', Acta Horticulturae, 961, pp. 167-174. International Society of Horticultural Science: Retrieved from https://pearl.plymouth.ac.uk/bms-research/1826
