ORCID
- Aljafer, Naofel: 0000-0003-2150-7698
Abstract
Pectinase enzymes are important industrial enzymes having considerable applications in several industries, especially in food processing. Pectinases contribute 25% of global food enzyme sales. Therefore, the demand for a commercial enzyme with desirable characteristics and low production costs has become one of the great targets. Hence, this study aims to produce exo-polygalacturonase (exo-PG) using local fungal isolate Penicillium oxalicum AUMC 4153 by utilizing sugar beet manufacturing waste (sugar beet pulp) as a sole raw carbon source under shaken submerged fermentation, which is purified and characterized to optimize enzyme biochemical properties for industrial application. The purity of the obtained exo-PG was increased by about 28-fold, and the final enzyme yield was 57%. The partially purified enzyme was active at a broad range of temperatures (30–60 °C). The optimum temperature and pH for the purified exo-PG activity were 50 °C and pH 5. The enzyme was stable at a range of pH 3 to 6 and temperature 30–50 °C for 210 min. The values for Km and Vmax were 0.67 mg/mL, with polygalacturonic acid as substrate and 6.13 µmole galacturonic acid/min/mg protein, respectively. It can be concluded that purified exo-PG production by P. oxalicum grown on sugar beet waste is a promising effective method for useful applications.
DOI
10.3390/life12020284
Publication Date
2022-02-14
Publication Title
Life
Volume
12
Issue
2
Embargo Period
2022-03-12
Organisational Unit
School of Biological and Marine Sciences
Recommended Citation
Almowallad, S. A., Alshammari, G., Alsayadi, M., Aljafer, N., Al-Sanea, E., Yahya, M., & Al-Harbi, L. (2022) 'Partial Purification and Characterization of Exo-Polygalacturonase Produced by Penicillium oxalicum AUMC 4153', Life, 12(2). Available at: https://doi.org/10.3390/life12020284
