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dc.contributor.authorDrevinek, P
dc.contributor.authorBaldwin, A
dc.contributor.authorLindenburg, L
dc.contributor.authorjoshi, lovleen
dc.contributor.authorMarchbank, A
dc.contributor.authorVosahlikova, S
dc.contributor.authorDowson, CG
dc.contributor.authorMahenthiralingam, E
dc.date.accessioned2017-08-16T16:35:48Z
dc.date.available2017-08-16T16:35:48Z
dc.date.issued2010-01
dc.identifier.issn0095-1137
dc.identifier.issn1098-660X
dc.identifier.urihttp://hdl.handle.net/10026.1/9839
dc.description.abstract

<jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Burkholderia cenocepacia</jats:italic> can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the Czech Republic experienced an epidemic outbreak caused by a <jats:italic>B. cenocepacia</jats:italic> ST-32 strain. The clonality of the isolates was evident by multilocus sequence typing; however, fingerprinting profiles obtained by pulsed-field gel electrophoresis (PFGE) showed substantial band variability. We investigated whether the PFGE pattern diversity resulted from genomic rearrangements mediated by insertion sequences (IS); in addition, we determined whether stressful growth conditions altered the transposition activity of these IS. DNA probes for IS commonly found in <jats:italic>B. cenocepacia</jats:italic> were designed using the <jats:italic>B. cenocepacia</jats:italic> J2315 genome. Southern hybridization analysis of ST-32 isolates demonstrated diversity in both the copy number and the insertion site for a homologue of ISBcen20. Movement of the ISBcen20 homologue was detected when the ST-32 isolate CZ1238 was exposed to oxidative stress (growth in the presence of H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> ). PFGE analysis of CZ1238 derivatives exposed to oxidative stress demonstrated genomic rearrangements. Interestingly, when the closely related <jats:italic>B. cenocepacia</jats:italic> strain J2315 was exposed to oxidative stress, no movement of ISBcen20 was detected. Since frameshift mutations are present within the transposases of all copies of this IS in J2315, our data suggest that the transposase is inactive. In summary, we have demonstrated for the first time that IS movement can be mediated by oxidative stress and can lead to genomic rearrangements in the CF pathogen <jats:italic>B. cenocepacia</jats:italic> . These IS movements may alter the PFGE fingerprints of isolates that are clonal by other typing methods. </jats:p>

dc.format.extent34-40
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.subjectBacterial Typing Techniques
dc.subjectBurkholderia
dc.subjectBurkholderia Infections
dc.subjectCluster Analysis
dc.subjectCystic Fibrosis
dc.subjectCzech Republic
dc.subjectDNA Fingerprinting
dc.subjectDNA Transposable Elements
dc.subjectDNA, Bacterial
dc.subjectDisease Outbreaks
dc.subjectElectrophoresis, Gel, Pulsed-Field
dc.subjectGenetic Variation
dc.subjectGenotype
dc.subjectHumans
dc.subjectMolecular Epidemiology
dc.subjectOxidative Stress
dc.subjectRecombination, Genetic
dc.titleOxidative Stress of <i>Burkholderia cenocepacia</i> Induces Insertion Sequence-Mediated Genomic Rearrangements That Interfere with Macrorestriction-Based Genotyping
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000276151500003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue1
plymouth.volume48
plymouth.publication-statusPublished
plymouth.journalJournal of Clinical Microbiology
dc.identifier.doi10.1128/jcm.01433-09
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dc.identifier.eissn1098-660X
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1128/jcm.01433-09
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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