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dc.contributor.authorjoshi, lovleen
dc.contributor.authorPhillips, DS
dc.contributor.authorWilliams, CF
dc.contributor.authorAlyousef, A
dc.contributor.authorBaillie, L
dc.date.accessioned2017-08-16T16:30:56Z
dc.date.available2017-08-16T16:30:56Z
dc.date.issued2012-11
dc.identifier.issn0099-2240
dc.identifier.issn1098-5336
dc.identifier.urihttp://hdl.handle.net/10026.1/9836
dc.description.abstract

<jats:title>ABSTRACT</jats:title> <jats:p> <jats:named-content content-type="genus-species">Clostridium difficile</jats:named-content> is the commonest cause of hospital-acquired infection in the United Kingdom. We characterized the abilities of 21 clinical isolates to form spores; to adhere to inorganic and organic surfaces, including stainless steel and human adenocarcinoma cells; and to germinate. The composition of culture media had a significant effect on spore formation, as significantly more spores were produced in brain heart infusion broth (Student's <jats:italic>t</jats:italic> test; <jats:italic>P</jats:italic> = 0.018). The spore surface relative hydrophobicity (RH) varied markedly (14 to 77%) and was correlated with the ability to adhere to stainless steel. We observed no correlation between the ribotype and the ability to adhere to steel. When the binding of hydrophobic (DS1813; ribotype 027; RH, 77%) and hydrophilic (DS1748; ribotype 002; RH, 14%) spores to human gut epithelial cells at different stages of cell development was examined, DS1813 spores adhered more strongly, suggesting the presence of surface properties that aid attachment to human cells. Electron microscopy studies revealed the presence of an exosporium surrounding DS1813 spores that was absent from spores of DS1748. Finally, the ability of spores to germinate was found to be strain and medium dependent. While the significance of these findings to the disease process has yet to be determined, this study has highlighted the importance of analyzing multiple isolates when attempting to characterize the behavior of a bacterial species. </jats:p>

dc.format.extent7671-7679
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.subjectAdenocarcinoma
dc.subjectBacterial Adhesion
dc.subjectCaco-2 Cells
dc.subjectCell Line, Tumor
dc.subjectClostridioides difficile
dc.subjectEpithelial Cells
dc.subjectGastrointestinal Tract
dc.subjectHumans
dc.subjectHydrophobic and Hydrophilic Interactions
dc.subjectIntestinal Mucosa
dc.subjectRibotyping
dc.subjectSpores, Bacterial
dc.subjectStainless Steel
dc.subjectSurface Properties
dc.titleContribution of Spores to the Ability of Clostridium difficile To Adhere to Surfaces
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000309678600021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue21
plymouth.volume78
plymouth.publication-statusPublished
plymouth.journalApplied and Environmental Microbiology
dc.identifier.doi10.1128/aem.01862-12
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dc.identifier.eissn1098-5336
dc.rights.embargoperiodNo embargo
rioxxterms.versionofrecord10.1128/aem.01862-12
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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